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01696 Sigma-Aldrich

Acrylamide

for Northern and Southern blotting, powder blend

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein Registry Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  PubChem Substance ID 329747346

  •  eCl@ss 39031205

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Properties

grade   for molecular biology
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
InChI Key   HRPVXLWXLXDGHG-UHFFFAOYSA-N
product line   BioReagent
assay   ≥99.5% (GC)
form   powder blend
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
bp   125 °C/25 mmHg(lit.)
mp   82-86 °C(lit.)
solubility   H2O: soluble0.25 g/mL at 20 °C, clear, colorless
storage temp.   2-8°C

Description

Application

Suitable for making polyacrylamide gels according to established recipes.

Caution

Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
3
RTECS 
AS3325000
Flash Point(F) 
280.4 °F
Flash Point(C) 
138 °C
Protocols & Articles

Articles

Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Peer-Reviewed Papers
15

References

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