01696 Sigma-Aldrich


for Northern and Southern blotting, powder blend

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein Registry Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  PubChem Substance ID 329747346

  •  eCl@ss 39031205



grade   for molecular biology
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
product line   BioReagent
assay   ≥99.5% (GC)
form   powder blend
impurities   DNases, none detected
  RNases, none detected
  phosphatases, none detected
  proteases, none detected
bp   125 °C/25 mmHg(lit.)
mp   82-86 °C(lit.)
solubility   H2O: soluble0.25 g/mL at 20 °C, clear, colorless
storage temp.   2-8°C



Suitable for making polyacrylamide gels according to established recipes.


Acrylamide powder is highly toxic through inhalation and skin contact. Acrylamide is considered to be a probable carciongen in humans. Standard laboratory nitrile gloves and full sleeve lab coats should be worn when handling acrylamide in any form. Dust masks or working in a fume hood is advised when handling acrylamide powder.

General description

Acrylamide powder is a white, odorless, crystalline solid. It is commonly used in molecular biology labs as a cross linking agent to cast polyacrylamide gels for nucleic acid electrophoresis.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Safety & Documentation

Safety Information

Signal word 
UN 2074 6.1 / PGIII
WGK Germany 
Flash Point(F) 
280.4 °F
Flash Point(C) 
138 °C
Protocols & Articles


Introduction to Markers and Ladders

Sigma-Aldrich offers a variety of markers that aid size determination of samples separated by agarose and/or polyacrylamide gel electrophoresis. These products include markers for DNA, PCR fragments ...
Keywords: Digestions, Electrophoresis, Gel electrophoresis, PAGE, Polymerase chain reaction

Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Peer-Reviewed Papers


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