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03677 Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

BioUltra, for molecular biology, ≥99.0% (T)

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

  • CAS Number 6381-92-6

  • Empirical Formula (Hill Notation) C10H14N2Na2O8 · 2H2O

  • Molecular Weight 372.24

  •  Beilstein Registry Number 3900609

  •  EC Number 205-358-3

  •  PubChem Substance ID 24845544

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
product line   BioUltra
assay   ≥99.0% (T)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
pH   4.0-5.5 (25 °C, 0.1 M in H2O)
mp   248 °C (dec.)(lit.)
solubility   H2O: soluble0.1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤50 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤5 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   0.1 M in H2O
UV absorption   λ: 260 nm Amax: 0.145
  λ: 280 nm Amax: 0.025

Description

Application

Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dehydrate can be used to study molecular biology, biochemicals, solutions and reagents, biological buffers, DNA and RNA purification, inorganic salts, essential chemicals and chelators. Ethylenediaminetetraacetic acid disodium salt dehydrate has been used in a study to advance monitoring of illegal thyreostatic drugs in livestock. Ethylenediaminetetraacetic acid disodium salt dehydrate has also been used in a study for the determination of 232Th in seawater by ICP-MS after preconcentration and separation using a chelating resin.

Other Notes

Running-buffer component for non-denaturing or urea-containing denaturing polyacrylamide gels in nucleic acid electrophoresis

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory Tract
RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AH4410000
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C
Protocols & Articles

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Peer-Reviewed Papers
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