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03690 Sigma-Aldrich

Ethylenediaminetetraacetic acid solution

BioUltra, for molecular biology, pH 8.0, ~0.5 M in H2O

Synonym: EDTA solution

  • CAS Number 60-00-4

  • Empirical Formula (Hill Notation) C10H16N2O8

  • Molecular Weight 292.24

  •  Beilstein Registry Number 1716295

  •  MDL number MFCD00003541

  •  PubChem Substance ID 329747771

  •  eCl@ss 39030907

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Properties

Related Categories BioUltra, Biochemicals and Reagents, Chelators, Essential Chemicals, Ethylenediaminetetraacetic acid (EDTA) and EDTA Solutions,
grade   for molecular biology
InChI Key   KCXVZYZYPLLWCC-UHFFFAOYSA-N
product line   BioUltra
concentration   ~0.5 M in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.363
pH   8.0±0.2
cation traces   Al: ≤10 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
absorption   absorption/268 nm cut-off
λ   neat
UV absorption   λ: 280 nm Amax: ≤0.25

Description

General description

EDTA has been titrated to pH 8.0 with NaOH

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Application

Ethylenediaminetetraacetic acid solution can be used to study cell biology, molecular biology, bioactive small molecules, biochemicals, solutions and reagents, inorganic salts, chelators and ethylenediaminetetraacetic acid (EDTA) and EDTA solutions. Ethylenediaminetetraacetic acid solution has been used in a study to help elucidate the role of antigen retrieval in immunostaining of ethanol-fixed smears. Ethylenediaminetetraacetic acid solution has also been used in a study to assess the uptake and release of biotin-labeled fluorescein (b-FITC) as well as immobilization of biotin-labeled glucose oxidase (b-GOx) to the polyelectrolyte microcapsules with an avidin-poly(styrene sulfonate) (PSS) membrane.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2
Protocols & Articles
Peer-Reviewed Papers
15

References

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