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04577 Sigma-Aldrich

Trizma® base

tested according to Ph.Eur.

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol, Trometamolum

  • CAS Number 77-86-1

  • Linear Formula NH2C(CH2OH)3

  • Molecular Weight 121.14

  •  Beilstein Registry Number 741883

  •  EC Number 201-064-4

  •  MDL number MFCD00004679

  •  PubChem Substance ID 329747947

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Properties

Related Categories Acids & Bases, Bases, Chemical Synthesis, Organic Bases, Synthetic Reagents More...
description   aminopeptidase substrate
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
form   crystalline
pharmacopeia   testing conforms to Pharmacopeia
color   colorless to white
pH   10.5-12
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
solubility   water: soluble 678 g/L at 20 °C

Description

Other Notes

The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.

Application

Trizma®base has been used for slab electrophoresis. It has also been used for preparing transfer buffer as well as Tris-buffered saline for western blotting.

Biochem/physiol Actions

Trizma ®base, commonly known as tris is widely used as a biological buffer or as a component of buffer formulations like- Tris-EDTA (TE) buffer, Tris magnesium buffer or Tris-acetate-EDTA (TAE) buffer. It enhances the membrane permeability of cell membranes.

Legal Information

Trizma is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000
Protocols & Articles

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Peer-Reviewed Papers
15

References

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