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05040 Sigma-Aldrich

Agar

powder, for microbiology

Synonym: Agar-agar, Gum agar

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Properties

Related Categories Agars, Base Ingredients for Media, Microbiology More...
grade   for microbiology
form   powder
ign. residue   ≤6%
loss   ≤20% loss on drying
pH   5-8 (50 °C, 1.5% in H2O, 100 °C/15 min.)
transition temp   gel point ~35 °C (1.5% solution)
gel strength   >300 g/cm2 (1.5% gel)
solubility   H2O: soluble1.5% at 70 °C, clear (100 °C/15 min.)

Description

General description

Agar has been used as reagent for the following studies:
• Plant cell culture studies.
• As medium supplement for the growth of wild-type Arabidopsis thaliana (ecotype Columbia) and transgenic seeds.
• For the solidification of culture medium of cork oak somatic embryos.
• It may be used for incubation of single antibody-forming cell to investigate the plaque formation.
•  Agar suspension culture may be used for the analysis of cell transformation of palyoma virus.

Agar is a complex gelatinous carbohydrate, and is added to the LB broth (Lysogeny broth, Luria broth, Lennox broth, or Luria-Bertani medium), in order to form gel for bacteria to grow upon. LB Broth is the most widely used nutritionally rich medium, for the culture and growth of bacteria. There are several different formulations of Lb broth, but the composition is generally the same comprising of peptides and casein peptones, trace elements, mineral and vitamins.

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2
RTECS 
AW7950000

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Peer-Reviewed Papers
15

References

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