05471 Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, ≥97.0% (HPCE)

Synonym: Albumin bovine serum, BSA, Bovine albumin



Related Categories Albumin, Biochemicals and Reagents, Bovine Serum Albumins, Ethanol Fractionated, Proteins and Derivatives More...
assay   ≥97.0% (HPCE)
form   lyophilized powder
mol wt   mol wt ~66 kDa
solubility   H2O: slightly soluble
storage temp.   2-8°C


Other Notes

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Preparation Note

Albumin from bovine serum is slightly soluble in water.

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.


Albumin from bovine serum can be used as a standard compound utilized in the trace analysis of proteins by using postcolumn solution-phase digestion methodology and Electrospray Mass Spectrometric analysis of marker peptides.

Biochem/physiol Actions

Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA blocks vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It triggers the insulin dependent diabetes mellitus.

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety Information

WGK Germany 


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