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05480 Sigma-Aldrich

Bovine Serum Albumin

fraction V, lyophilized, ≥96% (GE)

Synonym: Albumin bovine serum, BSA, Bovine albumin

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Properties

assay   ≥96% (GE)
form   lyophilized powder
quality   lyophilized
mol wt   Mr ~67000
  mol wt ~66 kDa
purified by   heat shock fractionation
loss   ≤3% loss on drying
pH   6.5-7.5
solubility   H2O: 40 mg/mL clear to almost clear, slightly yellow
storage temp.   2-8°C

Description

Other Notes

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Preparation Note

Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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