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05491 Sigma-Aldrich

Albumin, Acetylated from bovine serum

BioReagent, for molecular biology, lyophilized powder, ≥90.0% (GE)

Synonym: Ac-BSA

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Properties

grade   for molecular biology
product line   BioReagent
assay   ≥90.0% (GE)
form   lyophilized powder
impurities   HIV I and HIVII, HCV and HBsAg, tested negative
solubility   H2O: slightly soluble
foreign activity   DNase, RNase, protease, and alkaline phosphatase, none detected
storage temp.   2-8°C

Description

Application

Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Albumin from bovine serum has been used for electron and immunoelectron microscopy. It has also been used for isolating and culturing neonatal rat cardiac ventricular myocytes.

Other Notes

In the preparation of a bromouridine conjugate in EIA using a monoclonal antibody against 5-bromo-2-deoxyuridine for the assessment of lymphoid cell proliferation

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Preparation Note

Albumin from bovine serum is slightly soluble in water.

from BSA by acetylation in order to inactivate nucleases.

Biochem/physiol Actions

Bovine serum albumin also referred to as BSA or Fraction V is a protein isolated from cows. BSA can block vacant binding sites in enzyme-linked immunosorbent assay (ELISA) in both poly-l-lysine (PLL)-treated and non-treated microwells. It can also trigger insulin dependent diabetes mellitus in humans.

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Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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