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09688 Sigma-Aldrich

Ammonium acetate

BioUltra, for molecular biology, ≥99.0% (calc. based on dry substance, T)

  • CAS Number 631-61-8

  • Linear Formula CH3CO2NH4

  • Molecular Weight 77.08

  •  Beilstein Registry Number 4186741

  •  EC Number 211-162-9

  •  MDL number MFCD00013066

  •  PubChem Substance ID 329748799

  •  eCl@ss 39021908

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Properties

Related Categories BioUltra, Molecular Biology, Molecular Biology Reagents, Optimization Reagents, Protein Structural Analysis,
grade   for molecular biology
InChI Key   USFZMSVCRYTOJT-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (calc. based on dry substance, T)
form   solid
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤2% water
ign. residue   ≤0.01% (as SO4)
pH   6.5-7.5 (25 °C, 1 M in H2O)
mp   110-112 °C (dec.)(lit.)
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5 mg/kg
  nitrate (NO3-): ≤10 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤2 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.015
  λ: 280 nm Amax: ≤0.010

Description

Other Notes

Alternative to sodium acetate in ethanol precipitation of nucleic acids. Prevents precipitation of deoxyribonucleoside triphosphates.; Useful for removal of untreated triphosphates from the products of reverse transcriptase, DNA polymerase or terminal polymerase-catalyzed reactions.

Application

Ammonium acetate can be used to study molecular biology, reagents, protein structural analysis, proteomics and x-ray crystallography. Ammonium acetate has been used in a study to develop an easy, rapid, low-cost and effective cetyltrimethylammonium bromide (CTAB)-ammonium acetate method for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Ammonium acetate has also been used in a study to develop and validate a simple and rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of 4-p-anisamidobutyric acid (ABA; or N-anysoyl-γ-aminobutiryc acid, N-anisoyl-GABA), a major active metabolite of aniracetam, in human plasma.

Price and Availability


Laboratory Gloves

Amplified Detection

Biomedical Applications
Safety & Documentation
Protocols & Articles
Peer-Reviewed Papers
15

References

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