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09688 Sigma-Aldrich

Ammonium acetate

BioUltra, for molecular biology, ≥99.0% (calc. based on dry substance, T)

  • CAS Number 631-61-8

  • Linear Formula CH3CO2NH4

  • Molecular Weight 77.08

  •  Beilstein Registry Number 4186741

  •  EC Number 211-162-9

  •  MDL number MFCD00013066

  •  PubChem Substance ID 24846389

  •  eCl@ss 39021908

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Properties

Related Categories BioUltra, Molecular Biology, Molecular Biology Reagents, Optimization Reagents, Protein Structural Analysis,
grade   for molecular biology
InChI Key   USFZMSVCRYTOJT-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.0% (calc. based on dry substance, T)
form   solid
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤2% water
ign. residue   ≤0.01% (as SO4)
pH   6.5-7.5 (25 °C, 1 M in H2O)
mp   110-112 °C (dec.)(lit.)
solubility   H2O: soluble1 M at 20 °C, clear, colorless
anion traces   chloride (Cl-): ≤5 mg/kg
  nitrate (NO3-): ≤10 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤5 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤5 mg/kg
  Bi: ≤5 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤2 mg/kg
  K: ≤50 mg/kg
  Li: ≤5 mg/kg
  Mg: ≤5 mg/kg
  Mn: ≤5 mg/kg
  Mo: ≤5 mg/kg
  Na: ≤50 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Sr: ≤5 mg/kg
  Zn: ≤5 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: ≤0.015
  λ: 280 nm Amax: ≤0.010

Description

Other Notes

Alternative to sodium acetate in ethanol precipitation of nucleic acids. Prevents precipitation of deoxyribonucleoside triphosphates.; Useful for removal of untreated triphosphates from the products of reverse transcriptase, DNA polymerase or terminal polymerase-catalyzed reactions.

Same product — same quality — now Sigma-Aldrich brand

Application

Ammonium acetate can be used to study molecular biology, reagents, protein structural analysis, proteomics and x-ray crystallography. Ammonium acetate has been used in a study to develop an easy, rapid, low-cost and effective cetyltrimethylammonium bromide (CTAB)-ammonium acetate method for high-quality RNA isolation from polysaccharide- and polyphenol-rich cotton tissues. Ammonium acetate has also been used in a study to develop and validate a simple and rapid high-performance liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) method for the determination of 4-p-anisamidobutyric acid (ABA; or N-anysoyl-γ-aminobutiryc acid, N-anisoyl-GABA), a major active metabolite of aniracetam, in human plasma.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.




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