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09691 Sigma-Aldrich

Ammonium acetate solution

BioUltra, for molecular biology, ~5 M in H2O

  • CAS Number 631-61-8

  • Linear Formula CH3COONH4

  • Molecular Weight 77.08

  •  Beilstein Registry Number 4186741

  •  MDL number MFCD00013066

  •  PubChem Substance ID 24846390

  •  eCl@ss 39021908

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Properties

Related Categories Alphabetical, BioUltra, BioUltra Buffers, Biochemicals and Reagents, Biological Buffers,
grade   for molecular biology
InChI Key   USFZMSVCRYTOJT-UHFFFAOYSA-N
product line   BioUltra
concentration   ~5 M in H2O
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
ign. residue   ≤0.01% (as SO4)
pH   6.2-7.5 (25 °C, 1 M in H2O)
density   1.07 g/mL at 20 °C
anion traces   chloride (Cl-): ≤5 mg/kg
  nitrate (NO3-): ≤10 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Al: ≤1 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤1 mg/kg
  Bi: ≤1 mg/kg
  Ca: ≤5 mg/kg
  Cd: ≤1 mg/kg
  Co: ≤1 mg/kg
  Cr: ≤1 mg/kg
  Cu: ≤1 mg/kg
  Fe: ≤1 mg/kg
  K: ≤20 mg/kg
  Li: ≤1 mg/kg
  Mg: ≤1 mg/kg
  Mn: ≤1 mg/kg
  Mo: ≤1 mg/kg
  Na: ≤20 mg/kg
  Ni: ≤1 mg/kg
  Pb: ≤1 mg/kg
  Sr: ≤1 mg/kg
  Zn: ≤1 mg/kg
λ   1 M in H2O
UV absorption   λ: 260 nm Amax: 0.015
  λ: 280 nm Amax: 0.01

Description

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Application

Ammonium acetate solution can be used to study molecular biology, biological buffers, reagents and DNA and RNA purification. Ammonium acetate solution has been used to study pharmacokinetic analysis of α and β epimers of glycyrrhetinic acid in rat plasma. Ammonium acetate solution has also been used in a study to develop a method for the simultaneous determination of aristolochic acids A and B in some Chinese herbals and traditional Chinese patent medicines.

Price and Availability


Available in ELITE Grade

Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Protocols & Articles

Protocols

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

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Peer-Reviewed Papers
15

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