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09743 Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

  • CAS Number 54827-17-7

  • Linear Formula [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight 240.34

  •  Beilstein Registry Number 2808541

  •  EC Number 259-364-6

  •  MDL number MFCD00007748

  •  PubChem Substance ID 329748813

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Properties

Related Categories 2002/61/EC, Additional Standards, Alpha Sort, Analytical Standards, Analytical/Chromatography,
grade   analytical standard
  standard for GC
InChI Key   UAIUNKRWKOVEES-UHFFFAOYSA-N
assay   ≥99.0% (GC)
shelf life   limited shelf life, expiry date on the label
mp   166-171 °C
  168-171 °C(lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

Standard for determining aromatic amines released from azo-dyes in commodities

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DV2300000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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