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09743 Sigma-Aldrich

3,3′,5,5′-Tetramethylbenzidine

standard for GC

Synonym: BM blue, Sure Blue TMB, TMB Blotting Plus, TMB substrate, TMB

  • CAS Number 54827-17-7

  • Linear Formula [-C6H2(CH3)2-4-NH2]2

  • Molecular Weight 240.34

  •  Beilstein Registry Number 2808541

  •  EC Number 259-364-6

  •  MDL number MFCD00007748

  •  PubChem Substance ID 329748813

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Properties

Related Categories 2002/61/EC, Additional Standards, Alpha Sort, Analytical Standards, Analytical/Chromatography,
grade   analytical standard
  standard for GC
InChI Key   UAIUNKRWKOVEES-UHFFFAOYSA-N
assay   ≥99.0% (GC)
shelf life   limited shelf life, expiry date on the label
mp   166-171 °C
  168-171 °C(lit.)

Description

Application

3,3′,5,5′-Tetramethylbenzidine (TMB) is a noncarcinogenic substitute (Ames test negative) for benzidine suitable as peroxidase substrate for use in ELISA procedures. The substrate produces a soluble end product that is pale blue in color and can be read spectrophotometrically at 370 or 620-650 nm. The TMB reaction may be stopped with 2 M H2SO4 (resulting in a yellow color), and read at 450 nm. A sensitive and specific reagent for the detection of blood, assay of hemoglobin, assay of peroxidases.

Other Notes

Standard for determining aromatic amines released from azo-dyes in commodities

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DV2300000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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