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10416 Sigma-Aldrich

8-Anilino-1-naphthalenesulfonic acid ammonium salt

technical, ≥90% (NT)

Synonym: 1,8-ANS NH4, ANSA, Ammonium 8-anilino-1-naphthalenesulfonate, N-Phenyl peri acid

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Building Blocks, Chemical Synthesis, Fluorescent Indicators and Probes,
InChI Key   IPBNQYLKHUNLQE-UHFFFAOYSA-N
grade   technical
assay   ≥90% (NT)
solubility   NaOH: soluble1 N
  H2O: soluble
  methanol: soluble
fluorescence   λex 388 nm; λem 470 nm in 0.1 M Tris, 0.2 M KCl, pH 9.0, BSA

Description

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Application

ANS forms an inclusion complex with cyclodextrin. Such model systems are useful to mimic biological recognition and can be studied by measuring the change in fluorescence of free-ANS to complexed-ANS. When ANS enters the hydrophobic core of cyclodestrin, it’s fluorescence increases . Utilized in the reagent phase of a sodium-selective fiber-optic sensor. The reagent phase also contains a copper(II) polyelectrolyte, which binds to ANSA in the absence of sodium and quenches the fluorescence. In the presence of sodium, ANSA forms a cationic complex creating ion-pairs, causing it to fluoresce . ANS is often incorporated into di-block polymers and can be released by changes in the local environment (i.e., temperature, pH, etc.) . ANS is commonly used as a fluorescence probe to investigate molecular assemblies of surfactants and amphiphilic polymers because a blue shift of the emission maximum indicates the fluorophore is located in less polar media . Fluorescent probe for protein studies using methodologies such as steady-state and dynamic fluorescence measurements .

Price and Availability

Suggested Laboratory Gloves


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Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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