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10981 Sigma-Aldrich

Polyvinyl alcohol mounting medium with DABCO®, antifading

pH 8.7

Synonym: PVA-DABCO®

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Embedding Media, Embedding and Mounting Media, Microscopy Reagents More...
refractive index   n20/D 1.377
pH   8.7
density   1.08 g/mL at 20 °C
suitability   in accordance for application
shipped in   wet ice
storage temp.   2-8°C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Physical form

Aqueous solution containing tris(hydroxymethyl)aminomethane, tris(hydroxymethyl)aminomethane hydrochloride, polyvinyl alcohol 22000, glycerol, and 1,4-diazabicyclo[2.2.2]octane

Legal Information

DABCO is a registered trademark of Air Products & Chemicals, Inc.

Price and Availability

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
Flash Point(F) 
212 °F
Flash Point(C) 
100 °C

Documents

Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Can clear finger nail polish be used to replace Polyvinyl alcohol mounting medium with DABCO®, Product 10981, to seal the edges of the coverslips?
Anecdotal evidence suggests that permanent mounting medias do a better job of stopping evaporation of the aqueous mounting media. Clear finger nail polish has been used by some labs for sealing the edges of the coverslip.  We are unaware of any formal studies which have been performed that would document which product gives the better performance.
How does the use of aqueous Polyvinyl alcohol mounting medium with DABCO®, Product 10981, with an anti-fade agent differ from the use of a standard aqueous mounting media?
The anti-fade agent used in product 10981 will result in an initial drop in the intensity of the fluorescence.  Once the drop in fluorescence has occurred, the remaining fluorescence is not prone to rapid photo bleaching.
Is it necessary to seal the slides after coverslipping?
No. The slides do not require sealing after coverslipping. However sealing the edges of the coverslip with a permanent mounting media such as DPX will prevent the gradual formation of air bubbles as the aqueous mounting media gradually dries.
Is Polyvinyl alcohol mounting medium with DABCO®, Product 10981, a permanent mounting media?
No. This is considered an aqueous mounting media.  Although an antifade agent is included in the formulation, the fluorescence will eventually fade.
Polyvinyl alcohol mounting medium with DABCO®, Product 10981, has gelled during refrigeration.  Is the product still usable?
Yes, the product is still usable after gelling.  The product will however need to be reheated to around 50 degrees Celsius or higher.  It may take some time for the product to turn back into a liquid.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles
Peer-Reviewed Papers
15

References

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