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131164 Sigma-Aldrich

Indigo carmine

certified by the Biological Stain Commission, Dye content 85 %

Synonym: Acid Blue 74, Indigo-5,5′-disulfonic acid disodium salt, Indigocarmine

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, G-H-I, Hematology and Histology, Stains & Dyes, A to Z,
grade   certified by the Biological Stain Commission
InChI Key   KHLVKKOJDHCJMG-QDBORUFSSA-L
composition   Dye content, 85%
absorption   λmax 608 nm

Description

Packaging

25, 100 g in glass bottle

Application

Indigo carmine has been used to investigate its effects on ozone production by neutrophils.

Biochem/physiol Actions

Indigo Carmine (IC), commonly known as acid blue 74 or food blue 1 or FD and C blue 2 is a blue synthetic dye. It is used as a redox indicator in analytical chemistry and as a microscopic stain in biology. IC is also employed as a photometric detector. IC is a highly toxic indigoid dye and is associated with various health hazards such as irritation to the gastrointestinal tract leading to nausea, vomiting, and diarrhea and irritation in the respiratory tract leading to coughing and shortness of breath. IC exposure might also cause skin and eye irritations to humans. IC dye spray is used for the diagnosis of dysplasia in ulcerative colitis and is considered to be a simple, feasible, and safe method.

Suitability

Used as a collagen stain and in procedures using red nuclear stains.

Price and Availability


Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
DU3000000

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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