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14460 Sigma-Aldrich

N,N′-Bis(acryloyl)cystamine

BioXtra, ≥97.0% (HPLC)

Synonym: 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide

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Properties

Related Categories Gel Cross-Linkers, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-PAGE More...
InChI Key   DJVKJGIZQFBFGS-UHFFFAOYSA-N
product line   BioXtra
assay   ≥97.0% (HPLC)
mp   121-125 °C
cation traces   Ca: ≤1000 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤50 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤2000 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
suitability   in accordance for electrophoresis test
storage temp.   2-8°C

Description

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

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Laboratory Gloves
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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A4929 N,N′-Bis(acryloyl)cystamine, BioReagent, suitable for electrophoresis

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