• USA Home
  • 14460 - N,N′-Bis(acryloyl)cystamine

EMAIL THIS PAGE TO A FRIEND
14460 Sigma-Aldrich

N,N′-Bis(acryloyl)cystamine

BioXtra, ≥97.0% (HPLC)

Synonym: 2,2′-(Bisacrylamino)diethyl disulfide, BAC, Bis(2-acrylamidoethyl) disulfide

Purchase

Properties

Related Categories Gel Cross-Linkers, Molecular Biology, Protein Electrophoresis, Proteomics, SDS-PAGE More...
InChI Key   DJVKJGIZQFBFGS-UHFFFAOYSA-N
product line   BioXtra
assay   ≥97.0% (HPLC)
mp   121-125 °C
cation traces   Ca: ≤1000 mg/kg
  Cd: ≤5 mg/kg
  Co: ≤5 mg/kg
  Cr: ≤5 mg/kg
  Cu: ≤5 mg/kg
  Fe: ≤5 mg/kg
  K: ≤50 mg/kg
  Mg: ≤50 mg/kg
  Mn: ≤5 mg/kg
  Na: ≤2000 mg/kg
  Ni: ≤5 mg/kg
  Pb: ≤5 mg/kg
  Zn: ≤5 mg/kg
suitability   in accordance for electrophoresis test
storage temp.   2-8°C

Description

Application

BAC is used as a reversible cross-linker for polyacrylamide gels, to simplify isolation of macromolecules separated on the gels. The disulfide linkage can be broken with a suitable reducing agent. A 1-g slice of a 4% (12:1) gel will dissolve in 5 mL of 100 mM DTT in TBE (pH 8.3) in less than 30 min.

BAC is used as a reversible cross-linker for polyacrylamide gels. The disulfide linkage can be broken with a suitable reducing agent. A 12:1 ratio of acrylamide:BAC is recommended for DNA purification. A one gram slice of a 4% gel will dissolve in 5 mL of 100 mM DTT, TBE solution (pH 8.3) in less than 30 min. The pH of 8.3 is optimum for rapid dissolution of the gel.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

replaced by

Product #

Description

Add to Cart

A4929 N,N′-Bis(acryloyl)cystamine, BioReagent, suitable for electrophoresis

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?