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14510 Sigma-Aldrich

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid

≥96.0% (HPLC)

Synonym: BAPTA, Ethylenedioxybis(o-phenylenenitrilo)tetraacetic acid

  • CAS Number 85233-19-8

  • Empirical Formula (Hill Notation) C22H24N2O10

  • Molecular Weight 476.43

  •  Beilstein Registry Number 5192406

  •  MDL number MFCD00036255

  •  PubChem Substance ID 329750514

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Properties

Related Categories Biochemicals and Reagents, Chelation/Complexation Compounds, Chelators and Ion Probes, Chelators and Ion Probes for Flow Cytometry, Chelators and Ion Probes for Microscopy,
InChI Key   FTEDXVNDVHYDQW-UHFFFAOYSA-N
assay   ≥96.0% (HPLC)
fluorescence   λex 251 nm; λem 363 nm (pH 8.0 Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels)
  λex 274 nm; λem 363 nm (With Calcium)
storage temp.   2-8°C

Description

Application

Highly selective calcium chelating reagent. Suitable for the spectrophotometric monitoring of Ca2+ levels

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′′-tetraacetic acid was used for the spectrophotometric monitoring of Ca2+ levels. It may be used as chelator of cytosolic Ca²+ to investigate the effect of methoxychlor on cytosolic free Ca²+ concentrations and apoptosis in HA59T human hepatoma cells.

General description

1,2-Bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid is a highly selective calcium chelating reagent. Effect of microinjected calcium-loaded 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid (CaBAPTA) on cell-to-cell diffusion of carboxyfluorescein has been studied in staminal hairs of Setcreasea purpurea. It is reported to be a metabolite of 1,2-bis(2-aminophenoxy)ethane-N,N,N′,N′-tetraacetic acid tetra(acetoxymethyl ester) (BAPTA-AM) in rat urine.

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Laboratory Gloves

Cross-Coupling Guide
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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