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15356 Sigma-Aldrich

Blotting-Nylon 66 membranes, type B, positive

for Northern and Southern blotting

Synonym: Nylon 66-Blotting membranes, type B

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Properties

Related Categories Membranes & Blotting Paper, Molecular Biology, Nylon 66 Membranes, Positively Charged, Protein Electrophoresis, Proteomics,
grade   for molecular biology
form   roll
surface coverage   surface (quaternary ammonium groups) ≥99%
pore size   0.45 μm pore size
storage temp.   room temp

Description

Application

for high-sensitive nucleic acid blotting, dot and lot blots. Particularly suitable for radioactive detection. Suitable for protein transfer and immunoblotting.

Suitable for:
Protein transfer
Radioactive detection of blotted proteins and nucleic acids
Sensitive dot and slot blots of nucleic acids

General description

Blotting nylon provided on a roll is a standard item used in transfer of protein or nucleic acids during Western, Southern and Northern procedures. This membrane is positively charged nylon cast on a polyester support. It comes as a roll sized 0.2m x 3m, and can be cut to any size.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability


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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Southern and Northern Blotting

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Peer-Reviewed Papers
15

References

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