EMAIL THIS PAGE TO A FRIEND
15604 Sigma-Aldrich

Urea

puriss., meets analytical specification of Ph. Eur., BP, USP, 99.0-100.5%, 99.0-101.0% (calc. on dry substance)

Synonym: Carbamide, Carbonyldiamide

Purchase

Properties

InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
grade   puriss.
assay   99.0-100.5%
  99.0-101.0% (calc. on dry substance)
form   solid
quality   meets analytical specification of Ph. Eur., BP, USP
impurities   alkalic reac. substances, complies
  ≤0.001% heavy metals (as Pb)
  ≤0.04% insoluble in ethanol
  ≤0.05% ammonium (NH4)
  ≤0.1% biuret(carbamylurea)
ign. residue   ≤0.1% (as SO4)
loss   ≤1.0% loss on drying, 105 °C, 1 h
mp   132-135 °C(lit.)
  132-135 °C
solubility   H2O: soluble480 g/L at 20 °C
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤10 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Fe: ≤0.5 mg/kg
suitability   in accordance for appearance of solution
  in accordance for identity

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles
Peer-Reviewed Papers
15

References

Set your institution to view full text papers.
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?