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16658 Sigma-Aldrich

X-GalNAc

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-β-D-galactopyranoside, 5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide, X-Galactosaminide

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Properties

Related Categories Biochemicals and Reagents, Chromogenic, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Galactosaminidase, N-acetyl-beta-D,
grade   for molecular biology
InChI Key   SUWPNTKTZYIFQT-LMXXTMHSSA-N
sterility   non-sterile
assay   ≥98.0% (TLC)
form   powder
mp   ≥220 °C(lit.)
solubility   DMF: soluble
  DMSO: soluble
suitability   suitable for β-galactosidase test
storage temp.   −20°C

Description

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for the detection of Candida albicans.

General description

5-Bromo-4-chloro-3-indolyl N-acetyl-β-D-galactosaminide (X-GalNAc)is a chromogenic substrate for for N-acetyl-β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Principle

When X-GalNAc is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of X-GalNAc turn blue in color, allowing for easy differentiation between lac+ and lac- colonies.

Price and Availability


Amplified Detection
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

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