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16669 Sigma-Aldrich

Magenta-Gal

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-6-chloro-3-indolyl-β-D-galactopyranoside, Mag-Gal

  • CAS Number 93863-88-8

  • Empirical Formula (Hill Notation) C14H15BrClNO6

  • Molecular Weight 408.63

  •  Beilstein Registry Number 1552602

  •  MDL number MFCD00210022

  •  PubChem Substance ID 329751317

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Properties

Related Categories Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Galactosidase, beta, Substrates by Enzyme More...
grade   for molecular biology
InChI Key   CHRVKCMQIZYLNM-RKQHYHRCSA-N
sterility   non-sterile
assay   ≥98.0% (HPLC)
form   powder
solubility   DMF: soluble
  DMSO: soluble
suitability   suitable for β-galactosidase test
storage temp.   −20°C

Description

Application

Suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Also suitable for use immunoblotting and immunocytochemical assays.

General description

Magenta-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Magenta-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Principle

When Magenta-Gal is hydrolyzed by β-galactosidase, the resulting product will form a red precipitate. Lac+ colonies grown in the presence of Magenta-Gal turn an intense red color, allowing for easy differentiation between lac+ and lac- colonies.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Introduction to Blue-White Screening

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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