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17170 Sigma-Aldrich

AEA Sporulation Broth (Base), modified

for microbiology

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Properties

Related Categories A - M, All Food Control Media (alphabetical), All Liquid Media/Broths, Clostridia, Media,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   ammonium acetate, 1.5 g/L
  biopeptone, 10 g/L
  disodium phosphate, 4.36 g/L
  magnesium sulfate.7H2O, 0.2 g/L
  monopotassium phosphate, 0.25 g/L
  yeast extract, 10 g/L
final pH   7.8±0.2 (25 °C)
suitability   nonselective for Clostridia

Description

Application

For early sporulation of Clostridium perfringens from foods.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Preparation Note

Supplements:
4 g/l raffinose (Cat. No. 83400)
930 mg/l sodium carbonate (Cat. No. 71351)
42.6 mg/l cobalt chloride (Cat. No. 60818)
20 mg/l sodium ascorbate (Cat. No. 11140)

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Clostridia Diagnostic

Clostridia are relatively large, gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and even can be killed by exposure...
Jvo Siegrist
AnalytiX Volume 7 Article 2
Keywords: Aerobic, Anaerobic, Clinical, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology, Pharmaceutical, Vitamins

Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
ivo.siegrist@sial.com
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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