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17953 Sigma-Aldrich

Fluorescent IEF-Marker pI 3.5

Synonym: IEF-Marker fluorescent pI 3.5

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Properties

Related Categories Analytical Standards, Analytical/Chromatography, Electrophoresis, Fluorescent IEF Markers, Physical Properties Standards,
fluorescence   λex 318 nm; λem 415 nm in 0.1 M citrate pH 3.5
storage temp.   2-8°C

Description

Application

Suitable as IEF-Marker for HPCE

Reconstitution

Dissolve the IEF-marker at 2 mg/ml in 5 mM HCl, 50% 2-propanol, sonicate for up to 10 min if necessary. Store at 4 °C (the solution is stable for approximately 6 months). For use in HPCE this stock solution should be diluted 1:100 in a suitable ampholyte solution (e.g. 2% ampholyte 3-10), UV-detection at 280 nm (20 °C). For use in IEF-gels the 2 mg/ml stock solution can be loaded directly onto the gel (max. 1 μl per lane).

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
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