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17975 Sigma-Aldrich

Ninhydrin reagent according to Stahl

for TLC derivatization

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Description

Application

Ninhydrin reagent may be used for the quantitative determination of total protein content in tissue hydrolysates.

General description

Ninhydrin reagent is mostly used for the detection of amino acids. It basically gives a characteristic purple color on reaction with a-amino acids and other amino compounds, provided the pH of the reaction is 5.0 or higher. At an acid pH of 0.3, the reaction of ninhydrin with ornithinine and proline results in a red color product. Ninhydrin reagent can stay stable at room temperature in the absence of nitrogen atmosphere for upto 1 month in the ethylene glycol-sodium acetate solvent system.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1120 - class 3 - PG 3 - Butanols
WGK Germany 
2
Flash Point(F) 
93.2 °F
Flash Point(C) 
34 °C

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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