18795 Sigma-Aldrich

Brucella Agar

for microbiology



Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Brucella,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 13 g/L
  casein peptone, 10 g/L
  D(+)-glucose, 1 g/L
  meat peptone, 10 g/L
  sodium chloride, 5 g/L
  yeast extract, 2 g/L
final pH   7.0±0.2 (25 °C)
suitability   Haemophilus species
  nonselective for Brucella
  nonselective for Listeria
  nonselective for Neisseria
  nonselective for Streptococcus



For the isolation and cultivation of Brucella species, in particular the pathogens B. melitensis, B. abortus and B. suis, from clinical material and comestibles of animal origin, modified according to Wundt.

Preparation Note

Supplements :
Brucella differential agar:
1 ml/l 2% basic fuchsin solution (Cat. No. 03968). or 2% solution from thionine acetate (Cat. No. 88930)

Brucella selective agar:
500 mg/l Bacitracin (Cat. No. 11702)
1 mg/l Polymyxin B sulfate (Cat. No. 81334)
100 mg/l Cycloheximide (Cat. No. 01811)
(and if required) 1.25 mg/l ethyl violet (Cat. No. 05025)

For isolation of Campylobacter species:
50 ml/l heat inactivated (H1138) horse serum
2 vial/l Campylobacter Growth Supplement (Cat. No. C2847)
50-70 ml/l defibrinated sheep blood

Price and Availability

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
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Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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