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198099 Sigma-Aldrich

Methyl violet 2B

certified by the Biological Stain Commission

Synonym: Basic Violet 1

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Properties

Related Categories Cell Biology, Certified Stains & Dyes, Hematology and Histology, M, Stains & Dyes, A to Z,
grade   certified by the Biological Stain Commission
InChI Key   JFTBTTPUYRGXDG-UHFFFAOYSA-N
composition   Dye content, 75%
mp   137 °C (dec.)(lit.)
absorption   λmax 584 nm

Description

General description

Methyl Violet 2B is a mixture of polymethylated (4,5 and 6) pararosaniline chlorides

Packaging

25, 100 g in glass bottle

Application

Methyl violet 2B has been used to stain human lung fibroblast cells (A549) in in vitro antiviral assay.

Biochem/physiol Actions

Methyl violet 2B is a cationic dye with a triphenylmethane structure. It acts as a selective dye and facilitates the differentiation of Klebsiella pneumoniae from Enterobacter aerogenes and other gram-negative organisms. Methyl violet 2B is harmful if inhaled, swallowed or absorbed through skin, contact may cause damage to skin and eyes. Hence, the dye removal is of great importance.

Suitability

Certified for use in Flemming triple stain with iodine for chromosomes; Newton′s crystal violet-iodine technique for chromatin and nucleoli; Lieb′s amyloid stain.

Price and Availability


All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3
RTECS 
GC8425000
Protocols & Articles
Peer-Reviewed Papers
15

References

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