228842 Sigma-Aldrich

Ethyl Violet

cationic triarylmethane dye

Synonym: Basic violet 4, Ethyl purple 6B



Related Categories Cell Biology, E-F, Hematology and Histology, Stains & Dyes, A to Z, Stains and Dyes More...
absorption   λmax 595 nm



25 g in glass bottle


Ethyl violet is a cationic triarylmethane dye that is the ethyl homolog of crystal violet. It is a general histological and pathological stain. Ethyl violet has been used to stain pancreatic tissue and elastin,1 in the detection of gram negative bacteria,2 and as a reagent for the extractive-spectrophotometric determination of anionic surfactants.3 Ethyl violet induces mitochondrial permeability.4 It has been used in the extraction and spectrophotometric determination of copper.5 Along with the anionic dye zincon, ethyl violet is used in counterion dye of proteins in SDS-polyacrylamide gel electrophoresis.6

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Safety & Documentation

Safety Information

WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Peer-Reviewed Papers


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1. An elastic tissue stain. G.C.W. Humberstone, F.D. Humberstone J. Med. Lab. Technol. 26, 99, (1969)


2. Ethyl violet. A selective dye for the isolation of gram-negative bacteria. Litsky, W., et al. Biotech. Histochem. 27, 229-32, (1952)

3. Solvent extraction-spectrophotometric determination of anionic surfactants with ethyl violet. Motomizu, S., et al. Anal. Chem. 54, 392-397, (1982)

4. Mitochondrial effects of triarylmethane dyes. Kowaltowski, A.J.. et al. J. Bioenerg. Biomembr. 31, 581-90, (1999)


5. Solvent extraction of copper as its ion association complex with ethyl violet and its application to the spectrophotometric determination of copper. Yamamoto, K. and Motomizu, S. Analyst 112, 1011-4, (1987)

6. Counterion dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis and tryptic gel digestion of stained protein for mass spectrometry. Cong, W.T., et al. Methods Mol. Biol. 869, 497-509, (2012)


Spectrophotometric determination of trace arsenic in water samples using a nanoparticle of ethyl violet with a molybdate-iodine tetrachloride complex as a probe for molybdoarsenate. Morita K and Kaneko E Anal. Chem. 78(22), 7682-8, (2006)


[Sonocatalytic degradation of ethyl violet in the presence of high dispersion TiO2 and the comparison of catalytic activity with suspension TiO2]. Wang J, Jiang YF, Zhang ZH, et al. Huan Jing Ke Xue 28(2), 363-7, (2007)


[Preparation of nanometer TiO2 doped with upconversion luminescence agent and investigation on degradation of ethyl violet using visible light]. Wang J, Wen FY, Zhang ZH, et al. Huan Jing Ke Xue 27(6), 1133-9, (2006)


Resonance Rayleigh scattering study of interaction of hyaluronic acid with ethyl violet dye and its analytical application. Luo HQ, Li NB, and Liu SP Biosens. Bioelectron. 21(7), 1186-94, (2006)


Surfactants in atmospheric aerosols. Latif MT and Brimblecombe P Environ. Sci. Technol. 38(24), 6501-6, (2004)


Investigation on photocatalytic degradation of ethyl violet dyestuff using visible light in the presence of ordinary rutile TiO2 catalyst doped with upconversion luminescence agent. Wang J, Zhang G, Zhang Z, et al. Water Res. 40(11), 2143-50, (2006)


Fabrication of porous TiO2 film on Ti foil by hydrothermal process and its photocatalytic efficiency and mechanisms with ethyl violet dye. Mai FD, Lee WL, Chang JL, et al. J. Hazard. Mater. 177(1-3), 864-75, (2010)


Solvent and concentration effects on the visible spectra of tri-para-dialkylamino-substituted triarylmethane dyes in liquid solutions. Oliveira CS, Branco KP, Baptista MS, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 58(13), 2971-82, (2002)


Effect of molecular structure on the performance of triarylmethane dyes as therapeutic agents for photochemical purging of autologous bone marrow grafts from residual tumor cells. Indig GL, Anderson GS, Nichols MG, et al. J. Pharm. Sci. 89(1), 88-99, (2000)


TOF-S-SIMS molecular depth profiling of organic bilayers using mechanical wear test methodology. De Mondt R, Van Vaeck L, Heile A, et al. Anal. Bioanal. Chem 393(8), 1917-21, (2009)


Background-free, fast protein staining in sodium dodecyl sulfate polyacrylamide gel using counterion dyes, zincon and ethyl violet. Choi JK, Tak KH, Jin LT, et al. Electrophoresis 23(24), 4053-9, (2002)


Adsorption of ethyl violet dye in aqueous solution by regenerated spent bleaching earth. Tsai WT, Chang YM, Lai CW, et al. J. Colloid. Interface Sci. 289(2), 333-8, (2005)


Photophysical, photochemical, and tumor-selectivity properties of bromine derivatives of rhodamine-123. Lacerda SH, Abraham B, Stringfellow TC, et al. Photochem. Photobiol. 81(6), 1430-8, (2005)


Effect of self-association and protein binding on the photochemical reactivity of triarylmethanes. Implications of noncovalent interactions on the competition between photosensitization mechanisms type I and type II. Bartlett JA and Indig GL Photochem. Photobiol. 70(4), 490-8, (1999)


Previsible silver staining of protein in electrophoresis gels with mass spectrometry compatibility. Jin LT, Li XK, Cong WT, et al. Anal. Biochem. 383(2), 137-43, (2008)


Treatment of direct blending dye wastewater and recycling of dye sludge. Xu XH, Li ML, and Yuan Y Molecules 17(3), 2784-95, (2012)


Barium hydroxide lime turns yellow after desiccation. Barth CD, Dunning MB, Bretscher L, et al. Anesth. Analg. 101(3), 748-52, table of contents, (2005)


Novel and sensitive methods for the determination of dopamine based on the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering quenching effects. Ma Y, Li NB, and Luo HQ Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 73(4), 747-51, (2009)


A novel and sensitive method for recognition and indirect determination of Al(III) in biological fluid based on the quenching of resonance Rayleigh scattering intensities of "Al(III)-EV-DNA" complexing system. Long X, Li D, Wang N, et al. Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 69(1), 142-7, (2008)


Resonance light scattering of 1-hydroxypyrene-ethyl violet-anionic surfactant system and its analytical application. Ou-Yang YF, Wang YS, Mi XW, et al. Anal. Sci. 23(5), 533-7, (2007)


Mitochondrial targeting for photochemotherapy. Can selective tumor cell killing be predicted based on n-octanol/water distribution coefficients? Belostotsky I, da Silva SM, Paez MG, et al. Biotech. Histochem. 86(5), 302-14, (2011)


Final report on the safety assessment of Basic Violet 1, Basic Violet 3, and Basic Violet 4. Diamante C, Bergfeld WF, Belsito DV, et al. Int. J. Toxicol. 28(6 Suppl 2), 193S-204S, (2009)


A visible dye-based staining method for DNA in polyacrylamide gels by ethyl violet. Cong WT, Zhu ZX, He HZ, et al. Anal. Biochem. 402(1), 99-101, (2010)


Beil. 13,759

Aldrich MSDS 1, 941:B / Corp MSDS 1 (1), 1666:C / FT-IR 2 (3), 4131:B / FT-IR 1 (2), 964:B / RegBook 1 (2), 2777:F / Sigma FT-IR 1 (2), 137:D / Stains and Dyes Ref, 325 / Structure Index 1, 441:A:5

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