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252859 Sigma-Aldrich

Tris(hydroxymethyl)aminomethane

ACS reagent, ≥99.8%

Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Trometamol

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Properties

Related Categories ACS Grade Buffers, Amino Alcohols, Biochemicals and Reagents, Biological Buffers, Buffers A to Z,
grade   ACS reagent
InChI Key   LENZDBCJOHFCAS-UHFFFAOYSA-N
assay   ≥99.8%
  99.8-100.1%
impurities   ≤0.005% insolubles
  ≤2% water
useful pH range   7 - 9
pKa (25 °C)   8.1
bp   219-220 °C/10 mmHg(lit.)
mp   167-172 °C(lit.)
cation traces   Fe: ≤5 ppm
  heavy metals (as Pb): ≤5 ppm
absorption   / passes test

Description

Other Notes

Easily compare specifications for Trizma products with the Trizma specification table.

Packaging

100, 500 g in poly bottle

Application

Tris(hydroxymethyl)aminomethane has been used as a buffer solution for lactate dehydrogenase assay, in situ hybridization procedure and protein extraction from cells.

General description

Tris(hydroxymethyl)aminomethane is mainly required for preparation of buffers at physiological range of 7.3 to 7.5. The prepared buffers are compatible with biological fluids. It is of importance to laboratories as a standard pH solution.

Price and Availability


Laboratory Gloves

Interactive Buffer Table
Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TY2900000
Protocols & Articles

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Peer-Reviewed Papers
15

References

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