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25668 Sigma-Aldrich

Chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 49:1, ≥99.5% (chloroform + isoamyl alcohol, GC)

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Properties

Related Categories BioUltra, Molecular Biology, Molecular Biology Reagents More...
grade   for molecular biology
InChI Key   BKHZIBWEHPHYAI-UHFFFAOYSA-N
product line   BioUltra
assay   ≥99.5% (chloroform + isoamyl alcohol, GC)
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
  ≤0.001% free alkali (as NH3)
cation traces   Al: ≤0.5 mg/kg
  Ba: ≤0.1 mg/kg
  Bi: ≤0.1 mg/kg
  Ca: ≤0.5 mg/kg
  Cd: ≤0.05 mg/kg
  Co: ≤0.02 mg/kg
  Cr: ≤0.02 mg/kg
  Cu: ≤0.02 mg/kg
  Fe: ≤0.1 mg/kg
  K: ≤0.5 mg/kg
  Li: ≤0.1 mg/kg
  Mg: ≤0.1 mg/kg
  Mn: ≤0.02 mg/kg
  Mo: ≤0.1 mg/kg
  Na: ≤0.5 mg/kg
  Ni: ≤0.02 mg/kg
  Pb: ≤0.1 mg/kg
  Sr: ≤0.1 mg/kg
  Zn: ≤0.1 mg/kg
λ   neat
UV absorption   λ: 260 nm Amax: 0.1
  λ: 280 nm Amax: 0.07

Description

Components

mixture of chloroform and isoamyl alcohol in the ratio of 49:1 (v/v)

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Application

Chloroform – isoamyl alcohol mixture is a deproteinizing reagent used in conjunction with phenol for improving the efficiency of RNA and DNA (nucleic acid) extractions.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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