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  • 27285 - Ethylenediaminetetraacetic acid disodium salt dihydrate

27285 Sigma-Aldrich

Ethylenediaminetetraacetic acid disodium salt dihydrate

meets analytical specification of Ph. Eur., BP, USP, FCC, 99.0-101.0%

Synonym: Disodium ethylenediaminetetraacetate dihydrate, EDTA disodium salt, EDTA-Na2, Edathamil, Edetate disodium salt dihydrate, Sequestrene Na2

  • CAS Number 6381-92-6

  • Empirical Formula (Hill Notation) C10H14N2Na2O8 · 2H2O

  • Molecular Weight 372.24

  •  Beilstein Registry Number 3900609

  •  EC Number 205-358-3

  •  PubChem Substance ID 24856480



Related Categories Analytical/Chromatography, Core Bioreagents, Enzyme Inhibitors, Essential Chemicals, Ethylenediaminetetraacetic acid (EDTA) and EDTA Solutions,
grade   BP (tested according to)
  Ph Eur (tested according to)
  meets USP testing specifications (tested according to)
reg. compliance   FCC (tested according to)
assay   99.0-101.0%
quality   meets analytical specification of Ph. Eur., BP, USP, FCC
impurities   residual solvents, complies
  ≤0.001% cyanide (CN)
  ≤0.001% heavy metals (as Pb)
  ≤0.1% nitrilotriacetic acid (HPLC) (HPLC)
loss   8.7-11.4% loss on drying, 200 °C, 3 h
pH   4-5 (20 °C, 5%)
mp   248 °C (dec.)(lit.)
solubility   water: soluble100 g/L at 20 °C
anion traces   chloride (Cl-): ≤100 mg/kg
  sulfate (SO42-): ≤500 mg/kg
cation traces   As: ≤1 mg/kg
  Ca:, in accordance
  Fe: ≤10 mg/kg
  Pb: ≤10 mg/kg



Chelator of divalent cations. Inhibits enzymes, such as metalloproteases, that require divalent cations for activity.

Ethylenediaminetetraacetic acid disodium salt dihydrate has been used along with Na2HPO4 and citric acid for preparing Na2EDTA-McIlvaine buffer. Aqueous solution of Ethylenediaminetetraacetic acid disodium salt dihydrate has also been used for photocatalytic hydrogen production

Biochem/physiol Actions

Ethylenediaminetetraacetic acid disodium salt dihydrate is a widely used chelator of divalent cations like Ca+2 and inhibits metal ion-dependent proteases such as calcium-dependent cysteine proteases. EDTA also acts as a chelator of the zinc ion in the active site of metalloproteases; thus it inhibits metalloproteases that requires zinc for activity. Additionally, EDTA prevents platelet aggregation and is a preferred anticoagulant for platelet counts.

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
Target organs 
Respiratory Tract
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
>212 °F
Flash Point(C) 
>100 °C
Protocols & Articles
Peer-Reviewed Papers


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