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27544 Sigma-Aldrich

Clostridial Differential Broth

for microbiology

Synonym: DRCM, Differential Reinforced Clostridial Broth



Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Liquid Media/Broths, Clostridia,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   ammonium ferric citrate, 0.5 g/L
  casein peptone, 5.0 g/L
  L-cysteine hydrochloride, 0.5 g/L
  D(+)-glucose, 1.0 g/L
  meat extract, 8.0 g/L
  meat peptone, 5.0 g/L
  resazurine sodium salt, 0.002 g/L
  sodium acetate, 5.0 g/L
  sodium disulfite, 0.5 g/L
  starch, 1.0 g/L
  yeast extract, 1.0 g/L
final pH   7.1±0.2 (25 °C)
suitability   nonselective and differential for Clostridia



For the MPN enumeration of all clostridia in food and other material acc. to Gibbs and Freame.

For the cultivation and identification of Vibrio species from foods.

Other Notes

Suitable for the detection of sulfite-reducing, spore-forming anaerobes (Clostridia).

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

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