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29222 Sigma-Aldrich

Thio-H

Synonym: 2-Amino-5-(5-phenyl-2-thienyl)phenol-N,N,O-triacetic acid tricesium salt

  • Empirical Formula (Hill Notation) C22H16Cs3NO7S

  • Molecular Weight 837.15

  •  Beilstein Registry Number 9244017

  •  MDL number MFCD03791074

  •  PubChem Substance ID 329753323

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Description

Features and Benefits

This is a new Calcium indicator with promising properties which provide an excellent addition to fluorescent Ca2+/Mg2+ indicators available already. It is a low-affinity Ca2+-indicators (Kd ca 55 μM). Such indicators are useful for determination of Ca2+ in intracellular compartments [or for monitoring steep increases in intracellular concentration of Ca2+ .
Compared to wellknown low-affinity Ca2+ indicators Thio-H is about three times brighter. This is based on strong molar absorption as well as high quantum yields. High fluorescent output allows low indicator concentrations to be used, which decreases the cation buffering effects of indicator. The indicator offers the interesting property of being both excitation and emission rationable, thus ratiomeric measurements are possible both in excitations as emission mode. Further spectroscopic data (0.1 M Tris, pH 8.0): low [Ca2+], Exc.max. 362 nm, Em.max. 500 nm; high [Ca2+], Exc.max. 332 nm, Em.max. 500 nm

Other Notes

Intracellular, ratiomeric, fluorescent Ca2+/Mg2+ indicator with high brightness

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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