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30911 Sigma-Aldrich

Denaturation solution for the isolation of RNA

BioUltra, for molecular biology

Synonym: RNA isolation, denaturation

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Properties

Related Categories Alphabetical, BioUltra, Biochemicals and Reagents, Chaotropic Agents, Denaturation,
grade   for molecular biology
product line   BioUltra
composition   guanidine thiocyanate, 4 M
  Na-citrate, 25 mM
  sarcosyl, 0.5%
impurities   DNases, none detected
  RNases, none detected
  insoluble matter, passes filter test
  phosphatases, none detected
  proteases, none detected
refractive index   n20/D 1.433
pH   5.5-7.0
anion traces   chloride (Cl-): ≤500 mg/kg
  sulfate (SO42-): ≤50 mg/kg
cation traces   Al: ≤2 mg/kg
  As: ≤0.1 mg/kg
  Ba: ≤2 mg/kg
  Bi: ≤2 mg/kg
  Ca: ≤10 mg/kg
  Cd: ≤2 mg/kg
  Co: ≤2 mg/kg
  Cr: ≤2 mg/kg
  Cu: ≤2 mg/kg
  Fe: ≤2 mg/kg
  K: ≤100 mg/kg
  Li: ≤2 mg/kg
  Mg: ≤2 mg/kg
  Mn: ≤2 mg/kg
  Mo: ≤2 mg/kg
  Ni: ≤2 mg/kg
  Pb: ≤2 mg/kg
  Sr: ≤2 mg/kg
  Zn: ≤2 mg/kg
λ   neat
UV absorption   λ: 280 nm Amax: 1

Description

Other Notes

Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction

Application

Denaturation solution for the isolation of RNA is a guanidine thiocyanate, sarcosyl based protein denaturization solution useful for the purification of mRNA.

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Supplemental Hazard Statements 
Contact with acids liberates very toxic gas.
RIDADR 
UN 1760 8 / PGIII
WGK Germany 
2

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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