318906 Sigma-Aldrich

Eosin Y solution

5 wt. % in H2O

Synonym: 2′,4′,5′,7′-Tetrabromofluorescein disodium salt, Acid red 87, Eosin yellowish solution



Related Categories Biochemicals and Reagents, Cell Biology, E-F, Fluorescein and Derivatives, Fluorescent Labels,
concentration   5 wt. % in H2O
density   1.015 g/mL at 25 °C
absorption   λmax 518 nm


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Frequently Asked Questions are available for this Product.


500 mL in glass bottle

Price and Availability

Tissue Diagnostics

All labs need water

Biomedical Applications
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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Is Product 318906 suitable for use in Hematoxylin and Eosin (H&E) staining procedures?
Product 318906 was not intended for use with H&E staining techniques. The dye content for the Eosin Y is 5% in water. For H&E staining, the concentration is typically 0.5 to 1.0 %. At 5 % dye content, the dye is much too concentrated for use with H&E staining. If used at such a high concentration, the cytoplasmic staining is likely to obscure the nuclear staining from the hematoxylin. A well stained H&E slide should show blue nuclear staining and typically at least 3 shades of Eosin staining. When used at 5% dye content, the cytoplasmic staining is likely to be a homogenous or monotone. The 5% Eosin solution has only one common application - determining sperm viability.  In a typical semen analysis, 5% Eosin Y is used in conjunction with 10% Nigrosin. This combination of dyes is used to judge the viability of the sperm. A standard H&E procedure is then used to judge the morphology of the sperm. Sperm counts are typically performed without the use of a dye solution. It is possible to consider a 5% Eosin solution as a 10X staining solution. The product, however, is not suitability tested for use in either a sperm viability procedure or a H&E staining procedure. The following products have been suitability tested for use in H&E staining procedures and are considered suitable for use in In Vitro Diagnostic procedures. They are the preferred products for H&E staining. Most are formulated with 0.5% Eosin Y. The exception is Eosin Y with Phloxine B. For this formulation both the Eosin Y and the Phloxine B are formulated at 0.1% dye content. Eosin Y solution, alcoholic HT110116, HT110132, HT110180, HT1101128 Eosin Y solution, aqueous HT110216, HT110232, HT110280, HT1102128 Eosin Y solution, alcoholic, with phloxine HT110316, HT110332, HT110380, HT1103128
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