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33247 Sigma-Aldrich

Urea

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.5%

Synonym: Carbamide, Carbonyldiamide

  • CAS Number 57-13-6

  • Linear Formula NH2CONH2

  • Molecular Weight 60.06

  •  Beilstein Registry Number 635724

  •  EC Number 200-315-5

  •  MDL number MFCD00008022

  •  PubChem Substance ID 24859899

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Properties

Related Categories Analytical Reagents, Analytical Reagents for General Use, Analytical/Chromatography, Puriss p.a. ACS, T-Z, Puriss p.a. ACS More...
grade   ACS reagent, reag. Ph. Eur.
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
grade   puriss. p.a.
assay   ≥99.5%
  99-101% (calc. on dry substance)
form   solid
impurities   alkalic reac. substances, complies
  ≤0.0005% heavy metals (as Pb)
  ≤0.05% ammonium (NH4)
  ≤0.1% biuret
ign. residue   ≤0.01% (as SO4)
loss   ≤1.0% loss on drying, 105°C, 1 h
mp   132-135 °C(lit.)
  132-135 °C
solubility   H2O: soluble480 g/L at 20 °C
density   1.335 g/mL at 25 °C(lit.)
anion traces   chloride (Cl-): ≤5 mg/kg
  sulfate (SO42-): ≤10 mg/kg
cation traces   Fe: ≤2 mg/kg
suitability   in accordance for appearance of solution
  in accordance for identity
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Urea has been used in the following studies:
• Synthesis of SnO2 nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.
• Protein electrophoretic studies.

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Price and Availability

Suggested Laboratory Gloves


Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.



Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000
Protocols & Articles
Peer-Reviewed Papers
15

References

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