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341088 Sigma-Aldrich

1,9-Dimethyl-Methylene Blue zinc chloride double salt

Dye content 80 %

Synonym: DMMB, Taylor’s Blue

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Properties

InChI Key   MKLBAKXCEQNMBC-UHFFFAOYSA-J
form   powder
composition   Dye content, 80%
impurities   <20% water
mp   250 °C (dec.)(lit.)
absorption   extinction/243-249 nm, H2O ≥10000
  extinction/285-291 nm, H2O ≥18000
  extinction/594-600 nm, H2O ≥25000
  extinction/646-652 nm, H2O ≥35000
  λmax 649 nm

Description

Packaging

1 g in glass bottle

Application

1,9-Dimethyl-Methylene Blue zinc chloride double salt has been used for the quantification of sulfated glycosaminoglycans.

DMMB is a cationic dye that specifically binds to sulfated glycosaminoglycans (sGAG) with an absorbance at ~525 nm. It has been used to quantitate glycosaminoglyans such as chondroitin sulfate, keratin sulfate, and heparan sulfate and sGAG chains in proteoglycans. One limitation to this method The sGAG-DMMB complex is unstable in solution and may precipitate. Precipitation of the complex was shown to improve stability.

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Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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