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36018 Sigma-Aldrich

Fehling’s reagent I for sugars

Reag. Ph. Eur., for determination of sugar, solution I: copper(II) sulfate

Synonym: Copper(II) sulfate solution, Cupric sulfate solution acc. to Fehling, Cupric sulfate standard

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Application Specific Reagents, Food & Beverage, Pharmacopoeia,
grade   Reag. Ph. Eur.
  for determination of sugar
InChI Key   ARUVKPQLZAKDPS-UHFFFAOYSA-L
concentration   17.6 g/L±0.1 g/L Cu
density   1.038-1.060 g/mL at 20 °C
suitability   in accordance for application

Description

General description

The principle behind Fehling′ test is basically based on the reducing property of monosaccharide and disaccharides, which in turn depends on the presence of free keto or an aldehyde group. This test is performed by adding a few drops of sugar solution to a mixture of Fehling′s A solution (i.e., CuSO4) and Fehling′s B solution (i.e., KOH and Na-K tartarate). Fehling′s solution gets reduced by aldose sugars to insoluble yellow or red colored cuprous oxide. Monosaccharides like glucose, galactose and fructose and disaccharides like lactose and maltose resond freely to Fehling′s test as they possess a free keto or an aldehyde group, whereas sucrose fails to answer the test as it does not possess a free aldehyde or an keto group. Hence, Fehling′s test is also used to distinguish reducing discaccharides like maltose from non-reducing sugars like sucrose.

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Laboratory Gloves
Safety & Documentation

Safety Information

Symbol 
GHS09  GHS09
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 3082 9 / PGIII
WGK Germany 
3
Protocols & Articles
Peer-Reviewed Papers
15

References

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