375810 Sigma-Aldrich

Periodic acid

ACS reagent, 99%



Related Categories ACS Grade Acids & Bases, Acids, Acids & Bases, Analytical Reagents, Analytical Reagents for General Use,
grade   ACS reagent
assay   99%
  99.0-101.0% (ACS specification)
impurities   ≤0.01% insolubles
  ≤0.01% other halogens
ign. residue   ≤0.01%
mp   122 °C(lit.)
anion traces   sulfate (SO42-): ≤0.01%
cation traces   Fe: ≤0.003%
  heavy metals: ≤0.005%


Other Notes

A -D suffix exists for administrative purposes only.
All -D packages are 100% the same product, same quality, same specification as the package sizes previously sold without a -D.


25, 100 g in glass bottle


Periodic acid may be used in the following reactions:
• Oxidation of alcohols to ketones and aldehydes.1,2
• Oxidation hydroxylamine derivatives.3
• Oxidation of arenes to quinones.4
• Deprotection of the dithio- and oxathio-derivatives.5
• Oxidative degradation of epoxidized natural rubber (ENR).6
• Oxidation of urazoles and bis-urazoles to triazolinediones.7
• Oxidative cleavage of glucosazone.8

General description

Periodic acid is an inorganic acid commonly used as an oxidizing agent. Periodic acid oxidation9 is one of the steps in the periodate-resorcinol assay, used in the determination of sialic acid.10 Periodic acid reacts with 1,2 glycol linkage of carbohydrates to form aldehydes which can be stained by Schiff’s reagent. Therefore periodic-acid-Schiff′s reagent (PAS) is used in histochemistry to determine carbohydrates in tissue sections.11,12

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Explore the New Analytical Standards Portal

Customers Also Viewed

Periodic acid

for electrophoresis, ≥99%

Periodic acid

99.999% trace metals basis

Periodic acid

ReagentPlus®, ≥99.0%

Periodic acid

puriss. p.a., ACS reagent, for oxidimetric titration, crystallized, ≥99.0% (RT)

Periodic acid solution

1 g/dL in deionized water

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 3085 8(5.1) / PGII
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles

Related Content

Molarity Calculator and Normality Calculator for Acids and Bases

The molarity calculator tool provides lab-ready directions describing how to prepare an acid or base solution of specified Molarity (M) or Normality (N) from a concentrated acid or base solution.  To...

Peer-Reviewed Papers


Set your institution to view full text papers.

1. Pyridinium chlorochromate catalyzed oxidation of alcohols to aldehydes and ketones with periodic acid. Hunsen M. Tetrahedron Lett. 46(10), 1651-1653, (2005)

2. A mild and efficient oxidation of alcohols to aldehydes and ketones with periodic acid catalyzed by chromium (III) acetylacetonate. Xu L and Trudell ML. Tetrahedron Lett. 44(12), 2553-2555, (2003)

3. Further Observations Concerning the Periodic Acid Oxidation of Hydroxylamine Derivatives1. Emery TF and. Neilands JB. J. Org. Chem. 27(3), 1075-1077, (1962)

4. Chromium (VI) oxide-catalyzed oxidation of arenes with periodic acid. Yamazaki S. Tetrahedron Lett. 42(19), 3355-3357, (2001)

5. Deblocking of dithioacetals and oxathioacetals using periodic acid under mild nonaqueous conditions. Shi XX, et al. Tetrahedron Lett. 37(25), 4331-4334, (1996)

6. Chemical degradation of epoxidized natural rubber using periodic acid: Preparation of epoxidized liquid natural rubber. Phinyocheep P, et al. J. Appl. Polym. Sci. 95(1), 6-15, (2005)

7. The first report on the catalytic oxidation of urazoles to their corresponding triazolinediones via in situ catalytic generation of Br+ using periodic acid or oxone®/KBr system. Zolfigol MA, et al. J. Mol. Catal. A: Chem. 270(1), 219-224, (2007)

8. The Action of Periodic Acid on Glucose Phenylosazone. Chargaff E and Magasanik B. J. Am. Chem. Soc. 69(6), 1459-1461, (1947)

10. The sialic acids. XI. A periodate-resorcinol method for the quantitative estimation of free sialic acids and their glycosides. Jourdian GW, Dean L, and Roseman S J. Biol. Chem. 246(2), 430-5, (1971)


11. Carbohydrate histochemistry studied by acetylation techniques: I. Periodic acid methods. McManus JFA and Cason JE. J. Exp. Med. 91(6), 651, (1950)

12. Histological and histochemical uses of periodic acid. McMANUS JF Stain Technol. 23(3), 99-108, (1948)


Cross-comparison of protein recognition of sialic acid diversity on two novel sialoglycan microarrays. Padler-Karavani V, Song X, Yu H, et al. J. Biol. Chem. 287(27), 22593-608, (2012)


Oxidized dextran as crosslinker for chitosan cryogel scaffolds and formation of polyelectrolyte complexes between chitosan and gelatin. Berillo D, Elowsson L, and Kirsebom H Macromol. Biosci. 12(8), 1090-9, (2012)


An alternative role of C1q in bacterial infections: facilitating Streptococcus pneumoniae adherence and invasion of host cells. Agarwal V, Ahl J, Riesbeck K, et al. J. Immunol. 191(8), 4235-45, (2013)


[Oxidation of carbohydrates by periodic acid]. Fujibayashi S Tanpakushitsu Kakusan Koso., Suppl:136-42, (1968)


[Synthesis and identification of rutin complete antigen and analysis its immunogenicity]. Bao XY, He FY, Zeng JL, et al. Zhongguo Zhong Yao Za Zhi 38(3), 397-401, (2013)


Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides. Nilsson J and Larson G Methods Mol. Biol. 951, 79-100, (2013)


Enhancement of the nanofibrillation of wood cellulose through sequential periodate-chlorite oxidation. Liimatainen H, Visanko M, Sirviö JA, et al. Biomacromolecules 13(5), 1592-7, (2012)


The GLIB technique for genome-wide mapping of 5-hydroxymethylcytosine. Pastor WA, Huang Y, Henderson HR, et al. Nat. Protoc. 7(10), 1909-17, (2012)


Glycoproteomics enabled by tagging sialic acid- or galactose-terminated glycans. Ramya TN, Weerapana E, Cravatt BF, et al. Glycobiology 23(2), 211-21, (2013)


Synthesis of 2-hydroxy-1,4-oxazin-3-ones through ring transformation of 3-hydroxy-4-(1,2-dihydroxyethyl)-β-lactams and a study of their reactivity. Mollet K, Goossens H, Piens N, et al. Chemistry 19(10), 3383-96, (2013)


A nano-structured material for reliable speciation of chromium and manganese in drinking waters, surface waters and industrial wastewater effluents. Abdolmohammad-Zadeh H and Sadeghi GH Talanta 94, 201-8, (2012)


Screening of polysaccharides for preparation of α-amylase conjugate to enhance stability and storage life. Jadhav SB and Singhal RS Carbohydr. Polym. 92(2), 1724-9, (2013)


Beneficial effects of a novel RAGE inhibitor on early diabetic retinopathy and tactile allodynia. Guangyuan Li et al Mol. Vis. 17, 3156-65, (2011)


A sensitive assay for palytoxins, ovatoxins and ostreocins using LC-MS/MS analysis of cleavage fragments from micro-scale oxidation. Selwood AI, van Ginkel R, Harwood DT, et al. Toxicon 60(5), 810-20, (2012)


A periodate-cleavable linker for functional proteomics under slightly acidic conditions: application for the analysis of intracellular aspartic proteases. Maurer A, Zeyher C, Amin B, et al. J. Proteome Res. 12(1), 199-207, (2013)


Periodate salts as pyrotechnic oxidizers: development of barium- and perchlorate-free incendiary formulations. Moretti JD, Sabatini JJ, and Chen G Angew. Chem. Int. Ed. Engl. 51(28), 6981-3, (2012)


Pre-administration of L-tryptophan improved ADR-induced early renal failure in mice. Arioka Y, Yamamoto Y, Hoshi M, et al. Life Sci. 91(3-4), 100-6, (2012)


Profiling glycol-split heparins by high-performance liquid chromatography/mass spectrometry analysis of their heparinase-generated oligosaccharides. Alekseeva A, Casu B, Torri G, et al. Anal. Biochem. 434(1), 112-22, (2013)


Glucan of a somatic hybrid mushroom, pfls1h: structural characterization and study of immunological activities. Sen IK, Maji PK, Behera B, et al. Int. J. Biol. Macromol. 53, 127-32, (2013)


Enabling Wittig reaction on site-specific protein modification. Han MJ, Xiong DC, and Ye XS Chem. Commun. (Camb.) 48(90), 11079-81, (2012)


Structure analysis and antimutagenic activity of a novel salt-soluble polysaccharide from Auricularia polytricha. Zhou J, Chen Y, Xin M, et al. J. Sci. Food Agric. 93(13), 3225-30, (2013)


Development of a hypersensitive periodate-cleavable amino acid that is methionine- and disulfide-compatible and its application in MHC exchange reagents for T cell characterisation. Amore A, Wals K, Koekoek E, et al. ChemBioChem. 14(1), 123-31, (2013)


Controlled immobilisation of active enzymes on the cowpea mosaic virus capsid. Aljabali AA, Barclay JE, Steinmetz NF, et al. Nanoscale 4(18), 5640-5, (2012)


Expulsion of Trichuris muris is associated with increased expression of angiogenin 4 in the gut and increased acidity of mucins within the goblet cell. D'Elia R BMC Genomics 10, 492, (2009)


Chemical modification of carbohydrates in tissue sections may unmask mucin antigens. Kirkeby S Biotech. Histochem. 88(1), 19-26, (2013)


Development of a compact chemiluminescence system coupled with capillary electrophoresis for carbohydrate analysis. Zhu J, Shu L, Wu M, et al. Talanta 93, 428-32, (2012)


Deglycosylation effect of the mammalian sperm maturation antigen (SMA2) on serological reaction and acrosome reaction. Das T and Chatterjee T Anim. Reprod. Sci. 133(3-4), 176-83, (2012)


Real-time monitoring of tyrosine hydroxylase activity using a plate reader assay. Vermeer LM, Higgins CA, Roman DL, et al. Anal. Biochem. 432(1), 11-5, (2013)


Synthesis of a spacer-linked derivative of heptopyranosyl(α1-3)heptopyranose expressed in lipooligosaccharide and lipopolysaccharide. Yamasaki R, Nagahara K, Kishimoto K, et al. Biosci. Biotechnol. Biochem. 76(4), 772-7, (2012)


Improved covalent immobilization of horseradish peroxidase on macroporous glycidyl methacrylate-based copolymers. Prodanović O, Prokopijević M, Spasojević D, et al. Appl. Biochem. Biotechnol. 168(5), 1288-301, (2012)


9. Periodic Acid Oxidation. Jackson EL. Organic Reactions 2011th ed.,, 341-375

Merck 14,7171

Fieser 1,815 / Fieser 2,313 / Fieser 3,220 / Fieser 4,374 / Fieser 5,508 / Fieser 10,304 / Fieser 13,238

Corp MSDS 1 (2), 2731:D / RegBook 1 (3), 3253:H

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?