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38434 Sigma-Aldrich

N,N′-Diisopropyl-O-(4-nitrobenzyl)isourea

for HPLC derivatization, ≥90% (CHN)

Synonym: 1,3-Diisopropyl-2-(4-nitrobenzyl)isourea, O-(4-Nitrobenzyl)-N,N′-diisopropylisourea, NBDI

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Derivatization Reagents, Derivatization Reagents HPLC, UV-VIS More...
grade   for HPLC derivatization
InChI Key   FYDMCLCYYCIDMZ-UHFFFAOYSA-N
assay   ≥90% (CHN)
mp   37-40 °C
absorption   Amax/275 nm 9600 (mol. abs.)(lit.)
storage temp.   2-8°C

Description

Other Notes

Chromogenic reagent used for liquid chromatographic analysis of carboxylic acids

Same product — same quality — now Sigma-Aldrich brand

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

NBDI was used as derivatizing agent in a simple HPLC analysis of organic acids, in order to determine the important acid present in port wine and grape musts.

General description

N,N′-Diisopropyl-O-(4-nitrobenzyl)isourea (NBDI) is a derivatizing reagent. It is a strong chromophoric labelling agent which reacts with free carboxylic acids forming p-nitrobenzyl esters in high yields. Thereby giving better sensitivity and selectivity.

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Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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