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38434 Sigma-Aldrich

N,N′-Diisopropyl-O-(4-nitrobenzyl)isourea

for HPLC derivatization, ≥90% (CHN)

Synonym: 1,3-Diisopropyl-2-(4-nitrobenzyl)isourea, O-(4-Nitrobenzyl)-N,N′-diisopropylisourea, NBDI

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Derivatization Reagents, Derivatization Reagents HPLC, UV-VIS More...
grade   for HPLC derivatization
InChI Key   FYDMCLCYYCIDMZ-UHFFFAOYSA-N
assay   ≥90% (CHN)
mp   37-40 °C
absorption   Amax/275 nm 9600 (mol. abs.)(lit.)
storage temp.   2-8°C

Description

Other Notes

Chromogenic reagent used for liquid chromatographic analysis of carboxylic acids

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

NBDI was used as derivatizing agent in a simple HPLC analysis of organic acids, in order to determine the important acid present in port wine and grape musts.

General description

N,N′-Diisopropyl-O-(4-nitrobenzyl)isourea (NBDI) is a derivatizing reagent. It is a strong chromophoric labelling agent which reacts with free carboxylic acids forming p-nitrobenzyl esters in high yields. Thereby giving better sensitivity and selectivity.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
GHS06  GHS06
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGII
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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