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410217 Sigma-Aldrich

2′,7′-Dichlorofluorescein

ACS reagent

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Cell Biology, D, Fluorescent Indicators and Probes,
grade   ACS reagent
InChI Key   VFNKZQNIXUFLBC-UHFFFAOYSA-N
color   orange, powder
mp   280 °C (dec.)(lit.)
solubility   alcohol: passes test
absorption   λmax 509 nm
suitability   passes test for adsorption indicator

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1, 10 g in glass bottle

Price and Availability


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All labs need water

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Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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What can be used to make a solution of 2′,7′-dichlorofluorescein?
2′,7′-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2′,7′-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
In what types of applications can 2′,7′-Dichlorofluorescein be used?
Some applications for use of 2′,7′-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2′,7′-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
What excitation /emission wavelengths are best for fluorescence detection of 2′,7′-Dichlorofluorescein?
2′,7′-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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Protocols & Articles
Peer-Reviewed Papers
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References

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