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  • 410217 - 2′,7′-Dichlorofluorescein

410217 Sigma-Aldrich


ACS reagent



Related Categories Alphabetical, Biochemicals and Reagents, Cell Biology, D, Fluorescent Indicators and Probes,
grade   ACS reagent
color   orange, powder
mp   280 °C (dec.)(lit.)
solubility   alcohol: passes test
absorption   λmax 509 nm
suitability   passes test for adsorption indicator


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1, 10 g in glass bottle

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What can be used to make a solution of 2′,7′-dichlorofluorescein?
2′,7′-Dichlorofluorescein is soluble at 25 mg/mL absolute ethanol with heating. 2′,7′-Dichlorofluorescein is also soluble in water at 0.1 mg/mL.
In what types of applications can 2′,7′-Dichlorofluorescein be used?
Some applications for use of 2′,7′-Dichlorofluorescein are listed below. This product can be used for the determination of carbohydrates by fluorescence densitometry after TLC.  See G. Gübitz et al., J. Chromatogr., 117, 337, (1976). 2′,7′-Dichlorofluorescein as also been used in the determination of H2O2 by chemiluminescence. See the following references by H. Watanabe et al. Anal. Sci. 2, 461, (1986) and T. Segawa et al. Anal. Sci. 6, 763, (1990). This product has also been used as a  fluorescent additive used in indirect fluorimetric detection in OTC-HPLC. See W.D. Pfeffer and E.S. Yeung, J. Chromatogr., 506, 401, (1990).
What excitation /emission wavelengths are best for fluorescence detection of 2′,7′-Dichlorofluorescein?
2′,7′-Dichlorofluorescein can be detected at an excitation wavelength at 504 nm and emission wavelength at 529 nm in 0.1 M Tris pH 8.0.
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