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439142 Sigma-Aldrich

Chloroform

for HPLC, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer

Synonym: Methylidyne trichloride, Trichloromethane

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Properties

Related Categories Analytical Reagents, Analytical/Chromatography, Chloroform, Chromatography Reagents & Solvents, HPLC Grade Solvents,
grade   for HPLC
vapor density   4.1 (vs air)
vapor pressure   160 mmHg ( 20 °C)
InChI Key   HEDRZPFGACZZDS-UHFFFAOYSA-N
assay   ≥99.8%
contains   0.5-1.0% ethanol as stabilizer
packaging   PVC-coated bottle of 4, 4x4 L
impurities   <0.01% water
evapn. residue   <0.0003%
color   APHA: ≤10
refractive index   n20/D 1.445(lit.)
bp   60.5-61.5 °C(lit.)
mp   −63 °C(lit.)
density   1.48 g/mL at 25 °C
  1.492 g/mL at 25 °C(lit.)
cation traces   Pb: ≤0.05 ppm
λ   H2O reference
UV absorption   λ: 245 nm Amax: 1.00
  λ: 255 nm Amax: 0.15
  λ: 260 nm Amax: 0.15
  λ: 270 nm Amax: 0.02
  λ: 290-400 nm Amax: 0.01

Description

Packaging

4, 4×4 L in PVC-coated bottle

Application

Chloroform was used as an organic phase in the methylene blue (MB) two-phase titration process for estimating the cationic surfactant content in high salinity brine.
It may be used as:
• Solvent for electropolymerization.
• Solvent for recrystallization.
• Extractant in solvent extraction process.

General description

Chloroform is a colorless, volatile chlorinated organic solvent whose vapors have a narcotic effect. It is susceptible to degradation with time, which can be suppressed by adding ethanol as a stabilizer. Since ethanol is added in higher concentration it leads to increase in polarity which may have a potential influence on some of the applications. Reports suggest that chloroform stabilized with ethanol reduces chances on creating artifacts during the chromatographic separation after extraction of anthracyclines from biological matrices.

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Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 1888 6.1 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles
Peer-Reviewed Papers
15

References

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