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459836 Sigma-Aldrich

Ethyl alcohol, Pure

200 proof, anhydrous, ≥99.5%

Synonym: Ethanol, absolute alcohol, non-denatured ethanol

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Properties

Related Categories Allium cepa (Onion), Anhydrous Solvents, Cell Biology, Citrus aurantium (Seville orange), Ephedra sinica,
grade   anhydrous
assay   ≥99.5%
availability   not available in (Sales restictions may apply)
concentration   200 proof
impurities   <0.005% water
evapn. residue   <0.0005%
color   colorless
bp   78.3 °C
mp   -114 °C
density   0.789 g/mL at 20 °C

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Other Notes

Pure-Pac® II containers require the Micromatic MacroValve coupler for dispensing solvents, Z560723.

Sales restrictions may apply

Packaging

1, 6×1, 2, 4×2 L in Sure/Seal™

20 L in Pure-Pac™ 2

200 L in Pure-Pac™ 1

200 L in Pure-Pac™ 2

View returnable container options.

100, 12×100, 500 mL in Sure/Seal™

1250 L in Pure-Pac™ 1

Application

Ethanol has been used:
• as a solvent for lovastatin.
• as a solvent during the synthesis of (S)-4-hydroxy-2-keto-4-(2′-pyridyl)butyrate.
• to fix tissue sections on a slide during laser capture microdissection (LCM).

General description

Pure ethyl alcohol is a non-denatured 200proof ethanol. Ethanol, a primary alcohol, is an important raw material for the synthesis of various compounds. It can be synthesized either from molasses by fermentation or by hydration of ethylene. It acts as a hydrogen source and reaction medium during the transfer hydrogenation of carbonyl compounds in the presence of sodium hydroxide. The impact of ethanol/water fumigation on the performance of diesel engines has been studied. Anhydrous grade solvents show extremely low percentage water and low residue levels.

Legal Information

Pure-Pac is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability


All labs need water

Refer a colleague - New Lab Start-Up program
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN1170 - class 3 - PG 2 - Ethanol
WGK Germany 
1
RTECS 
KQ6300000
Flash Point(F) 
57.2 °F
Flash Point(C) 
14 °C
Protocols & Articles

Protocols

Experienced User Protocol | GenElut Mammalian Genomic DNA Miniprep Kit

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: PAGE

GenElute 96 Well Blood Genomic DNA Purification Kit Protocol

The starting materials are lysed at room temperature in a lysis buffer which contains chaotropic salts in the presence of Proteinase K. The addition of ethanol ensures the appropriate conditions to b...
Keywords: Buffers, Cell disruption, Centrifugation, DNA purification, Digestions, Evaporation, Precipitation, Purification, Respiratory

GenElute 96 Well Tissue Genomic DNA Purification Kit Protocol

The starting material is lysed by incubation in a solution containing SDS and Proteinase K. Once lysis is complete, chaotropic salts are then added to further promote solubilization of macromolecules...
Keywords: Buffers, Cell disruption, Centrifugation, DNA purification, Evaporation, Polymerase chain reaction, Purification, Respiratory

GenElute Bacterial Genomic DNA Kit Protocol

Sigma's GenElute™ Bacterial Genomic DNA Kit provides a simple and convenient way to isolate pure DNA from a variety of cultured bacteria. The kit contains all of the reagents needed to isolate and pu...
Keywords: Amplification, Anticoagulants, Cell disruption, Centrifugation, Degradations, Digestions, Evaporation, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation

GenElute Bacterial Genomic DNA Kit, Experienced User Protocol

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information.

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

Sigma’s GenElute Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whol...
Keywords: AGE, Amplification, Anticoagulants, Cell disruption, Centrifugation, DNA purification, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sample preparations, Sequencing

GenElute Plant Genomic DNA Miniprep Kit Protocol (G2N10, G2N70, G2N350)

Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. The purified DNA is greater than 20 kb in length and can be used in...
Keywords: AGE, Amplification, Cell disruption, Centrifugation, Electrophoresis, Evaporation, Filtration, Gel electrophoresis, Molecular biology, Phase transitions, Polymerase chain reaction, Precipitation, Purification

GenElute™ Blood Genomic DNA Kit Protocol (NA2010, NA2020)

Sigma’s GenElute™ Blood Genomic DNA Kit provides a simple and convenient way to isolate pure genomic DNA from fresh or aged (older than 24 hours) whole blood. The kit combines the advantages of silic...
Keywords: AGE, Addition reactions, Amplification, Anticoagulants, Cell disruption, Centrifugation, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing

Peer-Reviewed Papers
15

References

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