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48900 Sigma-Aldrich

Giemsa Stain, Modified Solution

(for the staining (of cellular blood components and blood parasites))

Synonym: Azure eosin methylene blue, Giemsa solution

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Properties

Related Categories Cell Biology, G-H-I, Hematology and Histology, Microbiology, Microscopy Reagents and Kits,
InChI Key   NALREUIWICQLPS-UHFFFAOYSA-N
type   (for research use only)
shelf life   limited shelf life

Description

Application

Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th edition, Williams & Wilkins, 173 (1981); together with May-Grünwald Solution for pap staining according to Pappenheim.

Caution

store at 20°C

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Principle

When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.

Reconstitution

Fix 10 min. in methanol; air-dry; immerse 45 min. in Giemsa Solution; rinse with distilled water; air-dry

Physical form

Solution of azure B/azure II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6 % (w/w)

Price and Availability


All labs need water
Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1230 - class 3 - PG 2 - Methanol, solution
WGK Germany 
2
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C
Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Peer-Reviewed Papers
15

References

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