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49742 Sigma-Aldrich

N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone

for fluorescence, ≥98.0% (HPLC)

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Properties

Related Categories Alphabetical, Biochemicals and Reagents, Chymotrypsin, alpha, Detection, Enzyme Substrates,
grade   for fluorescence
InChI Key   ARQVOICOWZVNEP-QHCPKHFHSA-N
assay   ≥98.0% (HPLC)
mp   272 °C(lit.)
solubility   DMF: soluble
  DMSO: soluble
fluorescence   λex 398 nm; λem 440 nm in 0.1 M Tris pH 8.0 (fluoroescence decreases upon cleavage with α-chymotrypsin)
storage temp.   2-8°C

Description

Analysis Note

In addition to the emission maximum at 440 nm, there is a lower maxima at 463 nm.

Other Notes

Substrate for the continuous assay of α-chymotrypsin at wavelengths 450/570nm

Same product — same quality — now Sigma-Aldrich brand

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Application

N-(N-Glutaryl-L-phenylalanyl)-2-aminoacridone is a fluorogenic substrate used to assay proteases/peptidases such as α-chymotrypsin and trypsin.

Price and Availability

Safety & Documentation

Safety Information

WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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