49825 Sigma-Aldrich

DMACA Reagent

for microbiology

Synonym: 4-(Dimethylamino)-cinnamaldehyde solution

  • CAS Number 6203-18-5

  • Empirical Formula (Hill Notation) C11H13NO

  • Molecular Weight 175.23

  •  Beilstein Registry Number 2208002

  •  MDL number MFCD00007002

  •  PubChem Substance ID 24873183

  • Popular Documents:  Datasheet (PDF)  




Composition per 100 ml:
Dimethylaminocinnamaldehye 1 g
Hydrochloric acid (concentrated) 1.0 ml
distilled water 99.0 ml


Used to determine the ability of an organism to split indole from the tryptophan molecule.

Other Notes

Reagent for the rapid identification of Streptococci.1,2

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Suggested Laboratory Gloves

Laboratory GlovesThis substance has been tested against several types of hand protection for CE compliance. Click below to find the recommended gloves for handling this product.

Search for Answers, Not Just Materials

Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
Personal Protective Equipment 
UN 1789 8 / PGIII
WGK Germany 

Protocols & Articles

Peer-Reviewed Papers


Set your institution to view full text papers.

1. L.P. Gordon et al. J. Clin. Microbiol. 2, 238, (1987)

Multi-laboratory validation of a standard method for quantifying proanthocyanidins in cranberry powders. Prior, R.L., et al. J. Sci. Food Agric. 90, 1473-8, (2010)


Inhibition of nicotinoprotein (NAD+-containing) alcohol dehydrogenase by trans-4-(N,N-dimethylamino)-cinnamaldehyde binding to the active site. Piersma SR, Norin A, de Vries S, et al. J. Protein Chem. 22(5), 457-61, (2003)


Evidence for reactivity of serine-74 with trans-4-(N,N-dimethylamino)cinnamaldehyde during oxidation by the cytoplasmic aldehyde dehydrogenase from sheep liver. Loomes KM, Midwinter GG, Blackwell LF, et al. Biochemistry 29(8), 2070-5, (1990)


The Arabidopsis TDS4 gene encodes leucoanthocyanidin dioxygenase (LDOX) and is essential for proanthocyanidin synthesis and vacuole development. Abrahams S, Lee E, Walker AR, et al. Plant J. 35(5), 624-36, (2003)


Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay. Feliciano, R.P., et al. J. Agric. Food Chem. 60, 4578-85, (2012)


Identification and biochemical characterization of mutants in the proanthocyanidin pathway in Arabidopsis. Abrahams S, Tanner GJ, Larkin PJ, et al. Plant Physiol. 130(2), 561-76, (2002)


Densitometric determination of (+)-catechin and (-)-epicatechin by 4-dimethylaminocinnamaldehyde reagent. Glavnik V, Simonovska B, and Vovk I J. Chromatogr. A. 1216(20), 4485-91, (2009)


Aldehyde dehydrogenase. Covalent intermediate in aldehyde dehydrogenation and ester hydrolysis. Blatter EE, Abriola DP, and Pietruszko R Biochem. J. 282 ( Pt 2), 353-60, (1992)


Comparison of adult corn rootworm (Coleoptera: Chrysomelidae) sampling methods. Whitworth RJ, Wilde GE, Shufran RA, et al. J. Econ. Entomol. 95(1), 96-105, (2002)


Amino acid substitutions at position 47 of human beta 1 beta 1 and beta 2 beta 2 alcohol dehydrogenases affect hydride transfer and coenzyme dissociation rate constants. Stone CL, Bosron WF, and Dunn MF J. Biol. Chem. 268(2), 892-9, (1993)


Evaluation of parameters that affect the 4-dimethylaminocinnamaldehyde assay for flavanols and proanthocyanidins. Wallace TC and Giusti MM J. Food Sci. 75(7), C619-25, (2010)


Determination of total procyanidins in selected chocolate and confectionery products using DMAC. Payne MJ, Hurst WJ, Stuart DA, et al. J. AOAC Int. 93(1), 89-96, (2010)


Use of stains to detect fingermarks. Becue A, Moret S, Champod C, et al. Biotech. Histochem. 86(3), 140-60, (2011)


Determination of sulfadoxine concentrations in whole blood using C18 solid-phase extraction, sodium dodecyl sulfate and dimethylaminocinnamaldehyde. Green MD, Mount DL, and Todd GD Analyst 120(10), 2623-6, (1995)


Chemical enhancement of footwear impressions in urine on fabric. Farrugia KJ, Bandey H, Bleay S, et al. Forensic Sci. Int. 214(1-3), 67-81, (2012)


Novel substrates of yeast alcohol dehydrogenase-3. 4-dimethylamino-cinnamaldehyde and chloroacetaldehyde. Leskovac V, Trivić S, Zeremski J, et al. Biochem. Mol. Biol. Int. 43(2), 365-73, (1997)


Photophysics of 4-(N,N-dimethylamino)cinnamaldehyde/ alpha-cyclodextrin inclusion complex. Panja S and Chakravorti S Spectrochim. Acta. A. Mol. Biomol. Spectrosc. 58(1), 113-22, (2002)


Binding and incorporation of 4-trans-(N,N-dimethylamino) cinnamaldehyde by aldehyde dehydrogenase. Dryjanski M, Lehmann T, Abriola D, et al. J. Protein Chem. 18(6), 627-36, (1999)


A selective and sensitive kinetic method for the determination of procaine and benzocaine in pharmaceuticals. Carmona M, Silva M, and Pérez-Bendito D J. Pharm. Biomed. Anal. 10(2-3), 145-52, (1992)


Catalysis of dehydrogenation of 4-trans-(N,N-dimethylamino)cinnamaldehyde by aldehyde dehydrogenase. Pietruszko R, Lehmann T, Dryjanski M, et al. Chem. Biol. Interact. 130-132(1-3), 103-14, (2001)


p-dimethylaminocinnamaldehyde derivatization for colorimetric detection and HPLC-UV/vis-MS/MS identification of indoles. Porubsky PR, Scott EE, and Williams TD Arch. Biochem. Biophys. 475(1), 14-7, (2008)


Nuclei of plants as a sink for flavanols. Feucht W and Polster J Z. Naturforsch., C, J. Biosci. 56(5-6), 479-81, (2001)


Forensic identification of urine using the DMAC test: a method validation study. Ong SY, Wain A, Groombridge L, et al. Sci. Justice 52(2), 90-5, (2012)


An environmentally friendly reflectometric method for bumetanide determination in pharmaceuticals. Pollo F, Sequinel R, Rufino JL, et al. Anal. Sci. 25(7), 897-901, (2009)


Aldehyde dehydrogenase: aldehyde dehydrogenation and ester hydrolysis. Pietruszko R, Abriola DP, Blatter EE, et al. Adv. Exp. Med. Biol. 328, 221-31, (1993)


2. J.F. MacFaddin Biochemical Tests for Identification of Medical Bacteria, (2000)

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?