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51465 Sigma-Aldrich

Urea

tested according to Ph.Eur.

Synonym: Carbamide, Carbonyldiamide, Ureum

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Properties

Related Categories Analytical/Chromatography, Biochemicals and Reagents, Chaotropic Agents, Denaturation, Pharmacopoeia,
InChI Key   XSQUKJJJFZCRTK-UHFFFAOYSA-N
assay   98.5-101.5% (Dried Substance)
form   solid
pharmacopeia   testing conforms to Pharmacopeia
mp   132-135 °C(lit.)
solubility   H2O: soluble 480 g/L at 20 °C (completely)
density   1.335 g/mL at 25 °C(lit.)
Gene Information   human ... CA1(759), CA2(760)
rat ... Ppm1a(24666)

Description

Application

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Price and Availability


Laboratory Gloves
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
YR6250000

Documents

Certificate of Analysis

Protocols & Articles
Peer-Reviewed Papers
15

References

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