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60983 Sigma-Aldrich

Kovac’s reagent for indoles

for microbiology

Synonym: 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac

  • CAS Number 100-10-7

  • Empirical Formula (Hill Notation) C9H11NO

  • Molecular Weight 149.19

  •  Beilstein Registry Number 4132845

  •  MDL number MFCD00003381

  •  PubChem Substance ID 329759335

  • Popular Documents:  Datasheet (PDF)  

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Properties

Related Categories Aldehydes, Biochemical Reagents for Identification, Building Blocks, C9, Carbonyl Compounds,
grade   for microbiology
InChI Key   BGNGWHSBYQYVRX-UHFFFAOYSA-N
shelf life   limited shelf life, expiry date on the label
composition   n-butanol
  4-dimethylaminobenzaldehyde
  hydrochloric acid
refractive index   n20/D 1.417
storage temp.   2-8°C

Description

Application

In the presence of oxygen, some bacteria, like E. coli, are able to split tryptophan into indole and α-aminopropionic acid. This reagent is for detecting the indole and identifying the indole-positive and indole-negative microorganisms.

General description

Solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2920 3(8) / PGII
WGK Germany 
1
Flash Point(F) 
96.8 °F
Flash Point(C) 
36 °C
Protocols & Articles

Articles

Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
ivo.siegrist@sial.com
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

EZ-Product Family for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration, Separation

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Peer-Reviewed Papers
15

References

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