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60983 Sigma-Aldrich

Kovac’s reagent for indoles

for microbiology

Synonym: 4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac

  • CAS Number 100-10-7

  • Empirical Formula (Hill Notation) C9H11NO

  • Molecular Weight 149.19

  •  Beilstein Registry Number 4132845

  •  MDL number MFCD00003381

  •  PubChem Substance ID 24882019

  • Popular Documents:  Datasheet (PDF)  



Related Categories Aldehydes, Biochemical Reagents for Identification, Building Blocks, C9, Carbonyl Compounds,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   n-butanol
  hydrochloric acid
refractive index   n20/D 1.417
storage temp.   2-8°C



In the presence of oxygen, some bacteria, like E. coli, are able to split tryptophan into indole and α-aminopropionic acid. This reagent is for detecting the indole and identifying the indole-positive and indole-negative microorganisms.

General description

Solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Other Notes

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 2920 3(8) / PGII
WGK Germany 
Flash Point(F) 
96.8 °F
Flash Point(C) 
36 °C
Protocols & Articles


Clostridium perfringens, their Properties and their Detection

Clostridia are relatively large, Gram-positive, rod-shaped bacteria that can undergo only anaerobic metabolism. Most Clostridia cannot grow under aerobic conditions and can even be killed by exposure...
AnalytiX Volume 10 Article 4
Keywords: Aerobic, Anaerobic, Chlorinations, Degradations, Environmental, Fermentation, Germination, Gram stain, Indicators, Metabolism, Microbiology

Differentiation of Escherichia coli from coliforms

In August 2008, the discovery of E. coli-contaminated beef in the United States prompted a nationwide recall of beef. The source turned out to be one supplier that had a history of contamination of i...
Jvo Siegrist
AnalytiX Volume 8 Article 5
Keywords: Decarboxylations, Fermentation, Genetic, Indicators, Microbiology, Reductions

EZ-Fit™ Filtration Products for Bioburden Testing

The EZ family of products has been designed to optimize and streamline the bioburden workflow. The family includes:
Keywords: Filtration

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram. The Gram Stain allows the determination of morphology, dividing bacteria into tw...
Jvo Siegrist
Microbiology Focus Edition 1.4, Product Manager Microbiology.... ivo.siegrist@sial.com
Keywords: Antibiotics, Enzyme activity, Fermentation, Gram stain, Help, Indicators, Reductions

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

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