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62087 Sigma-Aldrich

Levine EMB Agar

for microbiology

Synonym: Eosin methylene Blue Lactose Agar according to Levine, Levine’s Eosin Methylene Blue Agar

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Properties

Related Categories A - M, All Dairy Media (alphabetical), All Food Control Media (alphabetical), All Solid Media/Agars, Candida,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   agar, 13.5 g/L
  dipotassium hydrogen phosphate, 2.0 g/L
  eosin yellowish, 0.4 g/L
  lactose, 10.0 g/L
  meat peptone, 10.0 g/L
  methylene blue, 0.065 g/L
final pH   7.0±0.2 (37 °C)
suitability   selective and differential for Candida
  selective and differential for Enterobacter
  selective and differential for Enterococcus
  selective and differential for Escherichia coli and Coliforms
  selective and differential for Klebsiella
  selective and differential for Proteus
  selective and differential for Streptococcus

Description

Application

Isolation and differentiation of E. coli and Enterobacter, and identification of Candida albicans according to Levine.

Other Notes

Isolation and identification of coagulase-positive staphylococci; Screening method to isolate E. coli from ground beef

Same product — same quality — now Sigma-Aldrich brand

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiological Control of Spices and Herbs

Cultures around the world rely on herbs and spices to add flavour and zest to food. Many spices, however, contain very high numbers of bacteria, making them a potent source for food spoilage and path...
Jvo Siegrist
AnalytiX Volume 10 Article 1
Keywords: Growth factors, Microbiology

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Yeasts

Yeast is one of the most important microorganisms known and utilised by mankind. Ancient Middle Eastern civilisations used the organism to bake bread and to produce mead, beer and wine.
Jvo Siegrist
Analytix 2009, 5, 7.
Keywords: Antibiotics, Enzyme-linked immunosorbent assay, Fermentation, Microbiology, Molecular biology, Polymerase chain reaction, Reproduction, Vitamins

Peer-Reviewed Papers
15

References

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