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62970 Sigma-Aldrich

Lysozyme from chicken egg white

dialyzed, lyophilized, powder, ~100000 U/mg

Synonym: Mucopeptide N-acetylmuramoylhydrolase, Muramidase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
form   powder
quality   dialyzed
  lyophilized
mol wt   single-chain mol wt 14.3 kDa
  Mr ~14600
storage temp.   2-8°C
Gene Information   chicken ... LYZ(396218)

Description

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Other Notes

Suitable for the hydrolysis of bacterial cell walls, mucopolysaccharides, mucopolypeptides or chitin; degradability of L. lactis subsp. cremoris H2 cell walls; Review

Unit Definition

1 U corresponds to the amount of enzyme which decreases the absorbance at 450 nm by 0.001 per minute at pH 7.0 and 25°C (Micrococcus luteus, ATCC 4698, as substrate)

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Price and Availability


Biomedical Applications
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
OL5989000

Frequently Asked Questions

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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