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63163 Sigma-Aldrich

Malachite Green Broth

for microbiology

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Properties

Related Categories A - M, All Liquid Media/Broths, Media, Media by Application, Media by Microorganism,
grade   for microbiology
shelf life   limited shelf life, expiry date on the label
composition   dipotassium hydrogen phosphate, 1.1 g/L
  malachite green oxalate, 0.03 g/L
  meat extract, 9 g/L
  meat peptone, 15 g/L
final pH   7.0±0.2 (25 °C)
suitability   selective for Pseudomonas

Description

Application

For the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.

Malachite Green Broth has been used for the growth of Pseudomonas aeruginosa from water samples.

General description

Malachite Green Broth is for the selective enrichment of Pseudomonas aeruginosa according to Habs and Kirschner.
The culture medium has been recommended by Schubert and Blum for the examination of ground-, surface-, drinking-, bathing and process-water. In addition, it is suitable for the examination of mineral and spring-water.

Price and Availability

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Protocols & Articles

Articles

Microbiology Introduction

An initial aim of all microbiologists is the reproducible growth of their microbial cultures, no matter whether the microorganisms are of natural origin or have been genetically engineered by man. Re...
Keywords: Absorption, Aerobic, Anaerobic, Antibiotics, Antimicrobials, Buffers, Culture media, Diffusion, Environmental, Filtration, Genetic, Growth factors, Metabolism, Phase transitions, Precipitation, Reductions, Respiration, Sample preparations, Vitamins

Microbiology Theory: Media Preparation

Microorganisms need nutrients, a source of energy and certain environmental conditions in order to grow and reproduce. In the environment, microbes have adapted to the habitats most suitable for thei...
Keywords: Culture media, Digestions, Environmental, Filtration, Growth factors, Indicators, Microbiology, Phase transitions, Precipitation, Vitamins

Pseudomonas Media and Tests

Pseudomonas are motile (one or more polar flagella), rod shaped and aerobe Gram-negative bacteria. They are found almost everywhere, in soil, water, plants and animals. In most cases it is not pathog...
Jvo Siegrist, Product Manager Microbiology, ivo.siegrist@sial.com
Analytix Volume 2007 Article 5
Keywords: Anaerobic, Antifungals, Biochemistry, Cosmetics, Diagnostic, Environmental, Fermentation, Glycolysis, Metabolism, Metabolites, Microbiology, Pentose phosphate pathway, Pharmaceutical, Respiratory

Peer-Reviewed Papers
15

References

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