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65548 Sigma-Aldrich

Methanol

for HPLC, gradient grade, ≥99.8% (GC)

Synonym: Methyl alcohol

  • CAS Number 67-56-1

  • Linear Formula CH3OH

  • Molecular Weight 32.04

  •  Beilstein Registry Number 1098229

  •  EC Number 200-659-6

  •  MDL number MFCD00004595

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Properties

Related Categories Methanol, Solvent by Type, Solvents More...
grade   for HPLC, gradient grade
vapor density   1.11 (vs air)
vapor pressure   410 mmHg ( 50 °C)
  97.68 mmHg ( 20 °C)
assay   ≥99.8% (GC)
autoignition temp.   725 °F
shelf life   limited shelf life, expiry date on the label
expl. lim.   36 %
impurities   ≤0.0005% free alkali (as NH3)
  ≤0.0025% free acid (as HCOOH)
  ≤0.02% water
  ≤1 ppb quinine fluor., 254 nm
  ≤1 ppb quinine fluor., 365 nm
evapn. residue   ≤0.001%
refractive index   n20/D 1.329(lit.)
  n20/D 1.329
bp   64.7 °C(lit.)
mp   −98 °C(lit.)
density   0.791 g/mL at 25 °C(lit.)
absorption   /235 nm <2 mAU
  /254 nm <1 mAU
λ   1 cm path, H2O reference
UV absorption   λ: 205 nm Amax: 1.0
  λ: 210 nm Amax: 0.5
  λ: 220 nm Amax: 0.2
  λ: 230 nm Amax: 0.1
  λ: 250 nm Amax: 0.015

Description

Other Notes

A -D suffix exists for administrative purposes only.
All -D packages are 100% the same product, same quality, same specification as the package sizes previously sold without a -D.

Application

Methanol (MeOH) may be used in the following studies:
• Colony forming unit-fibroblast assay of bone marrow mononuclear cells.
• As solvent for the preparation of extracts of hyphae of Aspergillus for the estimation of gliotoxin by reversed phase-HPLC.
• Immunofluorescence studies.
• To compose eluent for the ion-pair reverse-phase HPLC isolation of nucleotides and their decomposition products.
• As eluent in the HPLC estimation of malondialdehyde in plasma, which is an indicator of oxidative stress.

General description

Methanol is an alcohol. Its thermochemical conversion to C2-C10 hydrocarbons in the presence of shape-selective zeolites has been reported. Its oxidation on Ru-Pt catalyst system by ruthenium ad-atoms has been proposed.

Price and Availability

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN1230 - class 3 - PG 2 - Methanol
WGK Germany 
1
RTECS 
PC1400000
Flash Point(F) 
48.2 °F
Flash Point(C) 
9 °C
Protocols & Articles
Peer-Reviewed Papers
15

References

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